Bertschinger H U, Moon H W, Whipp S C
Infect Immun. 1972 Apr;5(4):595-605. doi: 10.1128/iai.5.4.595-605.1972.
Two enteropathogenic strains of Escherichia coli (EEC) differed from a nonenteropathogenic strain of E. coli (NEEC) in their association with porcine small intestinal epithelium. The EEC characteristically were found along villi from tip to base and contiguous to the brush border. They were not in crypts. In contrast, the NEEC characteristically remained in the central lumen near the tips of villi and was only occasionally contiguous to the brush border. No organisms were detected within epithelial cells. The difference in distribution between EEC and NEEC was apparent in ligated jejunal loops 45 min postexposure. The association between host and bacterial cells was most consistently demonstrated on frozen sections of intestine, as other histological techniques removed many bacteria. However, cellular details of the association were best demonstrated in chemically fixed tissues.
两种肠致病性大肠杆菌（EEC）菌株与一种非致病性大肠杆菌（NEEC）菌株在与猪小肠上皮的关联方面存在差异。EEC菌株的特征是沿着绒毛从顶端到基部都有发现，并且与刷状缘相邻。它们不在隐窝中。相比之下，NEEC菌株的特征是留在绒毛顶端附近的中央管腔中，并且只是偶尔与刷状缘相邻。在上皮细胞内未检测到细菌。暴露后45分钟，在结扎的空肠环中，EEC和NEEC之间的分布差异很明显。宿主与细菌细胞之间的关联在肠道冰冻切片上最能持续显示出来，因为其他组织学技术会去除许多细菌。然而，这种关联的细胞细节在化学固定的组织中显示得最好。
