University Seed Farm, Ladhowal, Punjab Agricultural University, Ludhiana, Punjab, India.
N Biotechnol. 2009 Nov 30;26(5):239-43. doi: 10.1016/j.nbt.2009.09.003. Epub 2009 Sep 15.
In the present study we describe the construction of a genetic linkage map for the Brassica rapa (AA) genome that will act as a key resource in undertaking future structural and functional genomic studies in B. rapa. A F(2) mapping population consisting of 48 F(2) individual plants developed following hybridization of 2 inbred lines Bathari mandi and IC 331817 was used to construct the map. The map comprises 53 SSR markers derived from 3 different public domain resources. Nine linkage groups along with a small subgroup were identified and designated as R(1)-R(9) through alignment and orientation using SSR markers in common with existing B. rapa reference linkage maps. The total length of the genetic linkage map was 354.6 cm with an average interval of 6.6 cm between adjacent loci. The length of linkage groups ranged from 28.0 cm to 44.2 cm for R(6) and R(1A), respectively. The number variability of markers in the 9 linkage groups ranged from 3 for R(6) to 10 for R(1). Of the 53 SSR markers assigned to the linkage groups, only 5 (9.4%) showed deviation from the expected segregation ratio. The development of this map is vital to the genome integration and genetic information and will enable the international research community to share resources and data for the improvement of B. rapa and other cultivated Brassica species.
在本研究中，我们描述了构建甘蓝型油菜（AA）基因组遗传连锁图谱的过程，该图谱将成为未来在甘蓝型油菜中进行结构和功能基因组学研究的重要资源。我们使用由 2 个自交系 Bathari mandi 和 IC 331817 杂交产生的 48 个 F2 个体组成的 F2 作图群体来构建图谱。该图谱包含 53 个 SSR 标记，来源于 3 个不同的公共领域资源。通过使用与现有的甘蓝型油菜参考连锁图谱共有的 SSR 标记进行比对和定向，我们确定了 9 个连锁群和 1 个小亚群，并分别命名为 R(1)-R(9)。遗传连锁图谱的总长度为 354.6cm，相邻标记之间的平均间隔为 6.6cm。连锁群 R(6)和 R(1A)的长度分别为 28.0cm 和 44.2cm。9 个连锁群中标记的数量变化范围从 R(6)的 3 个到 R(1)的 10 个。分配给连锁群的 53 个 SSR 标记中，只有 5 个（9.4%）偏离了预期的分离比。该图谱的开发对于基因组整合和遗传信息至关重要，它将使国际研究界能够共享资源和数据，以提高甘蓝型油菜和其他栽培芸薹属物种的水平。
