Krisanapun Chutwadee, Peungvicha Penchom, Temsiririrkkul Rungravi, Wongkrajang Yuvadee
Department of Physiology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
Nutr Res. 2009 Aug;29(8):579-87. doi: 10.1016/j.nutres.2009.07.006.
The objective of this study was to evaluate the antidiabetic effects of the aqueous extract derived from the Thai Abutilon indicum Sweet plant and to explore its effects on intestinal glucose absorption and insulin secretion. The authors hypothesized that the plasma glucose level could be reduced through the inhibition of glucose absorption and/or the enhancement of insulin secretion. Administration of the extract (0.5 and 1 g/kg body weight) in an oral glucose tolerance test led to a significant reduction in plasma glucose levels in 30 minutes after the administration in moderately diabetic rats, as compared with untreated rats (P < .05), and this was at a faster rate than the use of an antidiabetic drug, glibenclamide. The inhibition of glucose absorption through the small intestine was investigated using an everted intestinal sac. The results showed that the extract at concentrations of 0.156 to 5 mg/mL caused a reduction of glucose absorption in a dose response manner. The maximum response was noted at a dose of 2.5 mg/mL. The promotion of the extract on insulin secretion was confirmed by incubating beta cell of pancreatic islets and INS-1E insulinoma cells with the extract at 1 to 1000 microg/mL. These observations suggest that the aqueous extract from the A indicum plant has antidiabetic properties, which inhibited glucose absorption and stimulated insulin secretion. Phytochemical screening also revealed that the extract contained alkaloids, flavonoids, tannins, glycosides, and saponins that could account for the observed pharmacologic effects of the plant extract.
本研究的目的是评估泰国苘麻植物水提取物的抗糖尿病作用，并探讨其对肠道葡萄糖吸收和胰岛素分泌的影响。作者推测，血浆葡萄糖水平可通过抑制葡萄糖吸收和/或增强胰岛素分泌而降低。在口服葡萄糖耐量试验中，给中度糖尿病大鼠灌胃提取物（0.5和1 g/kg体重），与未处理的大鼠相比，给药后30分钟血浆葡萄糖水平显著降低（P <.05），且降低速度比使用抗糖尿病药物格列本脲更快。使用外翻肠囊研究提取物对小肠葡萄糖吸收的抑制作用。结果表明，浓度为0.156至5 mg/mL的提取物以剂量反应方式降低葡萄糖吸收。在2.5 mg/mL剂量时观察到最大反应。通过将胰岛β细胞和INS-1E胰岛素瘤细胞与1至1000 μg/mL的提取物一起孵育，证实了提取物对胰岛素分泌的促进作用。这些观察结果表明，苘麻植物水提取物具有抗糖尿病特性，可抑制葡萄糖吸收并刺激胰岛素分泌。植物化学筛选还显示，提取物含有生物碱、黄酮类化合物、单宁、糖苷和皂苷，这些成分可能解释了植物提取物所观察到的药理作用。
