Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.
Anal Bioanal Chem. 2009 Nov;395(5):1433-9. doi: 10.1007/s00216-009-3106-0. Epub 2009 Sep 11.
In this paper, we describe a facile approach--using silicate fibers immobilized with Eu(III) ions [Eu(III) fibers] as affinity probes--to rapidly sense tetracycline (TC) in complex samples. The fabrication of the Eu(III) fibers is straightforward: Simply immerse a silicate fiber into a glass tube containing Eu(III) and irradiate with microwaves (power, 900 W) for 30 s. The Eu(III) fibers selectively trap TC from aqueous samples via chelation of the beta-diketone functional group of TC with the Eu(III) center. Because the Eu(III)-TC complexes on the fibers are luminescent, as a result of intermolecular energy transfer from the TC moieties to the Eu(III) centers, they can be detected directly using a fluorophotometer. To accelerate the sensing process, we also used microwave irradiation (for only 15 s) to trap the TC molecules from the sample solutions onto the Eu(III) fibers. Furthermore, only a small volume (< 10 microL) of sample solution is required for these analyses. This approach allows TC to be detected in aqueous samples, with a detection limit of 50 nM.
在本文中，我们描述了一种简便的方法——使用固定有 Eu(III)离子的硅酸盐纤维作为亲和探针[Eu(III)纤维]，可快速检测复杂样品中的四环素(TC)。Eu(III)纤维的制备很简单：只需将硅酸盐纤维浸入含有 Eu(III)的玻璃管中，然后用微波照射（功率 900 W）30 秒。Eu(III)纤维通过 TC 的β-二酮官能团与 Eu(III)中心的螯合作用，从水样中选择性地捕获 TC。由于纤维上的 Eu(III)-TC 配合物具有荧光性，这是由于 TC 部分到 Eu(III)中心的分子间能量转移所致，因此可以直接使用荧光光度计进行检测。为了加速传感过程，我们还使用微波辐射（仅 15 秒）将 TC 分子从样品溶液中捕获到 Eu(III)纤维上。此外，这些分析仅需要 <10 μL 的少量样品溶液。该方法允许在水样中检测 TC，检测限为 50 nM。
