Hu Min-Shan, Li Zheng-Ke, Fang Ding-Zhi
Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 Sep;44(5):727-30, 735.
To investigate the role of the - 250G/A polymorphism in the promoter region of hepatic lipase gene (LIPC) on serum lipid profile and its interactions with a high-carbohydrate/low-fat (HC/LF) diet on serum lipid profiles in a young healthy Chinese population.
After a stabilization diet for seven days, fifty-six young healthy subjects (27 males, 29 females) were given the HC/LF diet for six days. The serum lipid profiles were analyzed of the twelve-hour fasting venous blood samples collected in the mornings of the first, the eighth and the fourteenth days. The concentrations of serum apolipoproteins were measured. The LIPC -250G/A polymorphism were analyzed.
At baseline, the female subjects with the GG genotype had significantly higher high-density lipoprotein cholesterol (HDL-C) (P= 0. 041) and apolipoprotein A- I (Apo A- I ) (P= 0. 020) than the male subjects with the same genotype. After the stabilization diet, the females had significantly higher HDL-C (GG genotype: P=0. 021, A carriers: P=0. 014) and Apo A-I (GG genotype: P= 0. 035, A carriers: P= 0. 006) than the males in all genotypes. After the HC/LF diet, the female A carriers had significantly higher total cholesterol (TC) (P= 0. 042) than the male A carriers, and the females had significantly higher Apo A- I than the males in all genotypes (GG genotype: P=0. 010, A carriers: P=0. 009). Compared with thosebefore the HC / LF diet , TC ( males with GG genotype : P = 0. 013 , male A carriers: P = 0. 000 ; females with GG genotype: P=0. 025, female A carriers: P=0. 048) and low-density lipoprotein cholesterol (LDL-C) (males with GG genotype: P = 0. 028, male A carriers: P = 0. 000; females with GG genotype: P= 0. 004, female A carriers: P=0. 001) significantly decreased after the diet in all the subjects. Triglycerides (TAG) (GG genotype: P=0. 006, A carriers: P= 0. 001) significantly increased in the females regardless of the genotype. However, only in the male A carriers, HDL-C (P= 0. 011) and Apo A- I (P= 0. 041) significantly increased after the diet.
The A allele at the LIPC -250G/A polymorphism is associated with the HC/LF diet induced HDL-C and Apo A-I concentration changes in the males.
探讨肝脏脂肪酶基因（LIPC）启动子区域-250G/A多态性对年轻健康中国人群血脂谱的作用，以及其与高碳水化合物/低脂（HC/LF）饮食在血脂谱方面的相互作用。
56名年轻健康受试者（27名男性，29名女性）在进行7天的稳定饮食后，给予HC/LF饮食6天。分析在第1天、第8天和第14天早晨采集的12小时空腹静脉血样本的血脂谱。测量血清载脂蛋白浓度。分析LIPC -250G/A多态性。
在基线时，具有GG基因型的女性受试者的高密度脂蛋白胆固醇（HDL-C）（P = 0.041）和载脂蛋白A-I（Apo A-I）（P = 0.020）显著高于具有相同基因型的男性受试者。在稳定饮食后，所有基因型的女性的HDL-C（GG基因型：P = 0.021，A携带者：P = 0.014）和Apo A-I（GG基因型：P = 0.035，A携带者：P = 0.006）显著高于男性。在HC/LF饮食后，女性A携带者的总胆固醇（TC）（P = 0.042）显著高于男性A携带者，并且所有基因型的女性的Apo A-I显著高于男性（GG基因型：P = 0.010，A携带者：P = 0.009）。与HC/LF饮食前相比，所有受试者在饮食后TC（GG基因型男性：P = 0.013，A携带者男性：P = 0.000；GG基因型女性：P = 0.025，A携带者女性：P = 0.048）和低密度脂蛋白胆固醇（LDL-C）（GG基因型男性：P = 0.028，A携带者男性：P = （此处原文有误，应为0.000）；GG基因型女性：P = 0.004，A携带者女性：P = 0.001）显著降低。无论基因型如何，女性的甘油三酯（TAG）（GG基因型：P = 0.006，A携带者：P = 0.001）显著升高。然而，仅在男性A携带者中，饮食后HDL-C（P = 0.011）和Apo A-I（P = 0.041）显著升高。
LIPC -250G/A多态性处的A等位基因与HC/LF饮食诱导的男性HDL-C和Apo A-I浓度变化相关。
