Zocchi M R, Poggi A, Heltai S, Villa A, Inverardi L, Vicari A, Sabbadini M G, Ferrarini M
Istituto Scientifico San Raffaele, Milan, Italy.
Clin Exp Immunol. 1990 Oct;82(1):108-13. doi: 10.1111/j.1365-2249.1990.tb05412.x.
In order to define the signal requirements for leukaemic T cell activation, the proliferation and interleukin-2 (IL-2) production of peripheral lymphocytes from a patient with a HTLV-I-, CD4+, CD45RA+ CD45RO+ CD25- T-CLL were evaluated after the delivery of different stimuli. Unlike resting CD4+ normal T lymphocytes that can be activated only by a two-signal stimulation, T-CLL cells proliferated and released IL-2 in response to a pair of anti-CD2 monoclonal antibodies (MoAbs) or concanavalin A (Con A) in the absence of both accessory cells (AC) and phorbol myristate acetate (PMA). The two stimuli were also able to induce CD25 expression within 12-20 h on the majority of T-CLL cells. A response to anti-CD3 and anti-CD28 MoAbs was detected only in the presence of PMA, similar to that observed in normal resting T lymphocytes matched for phenotype. Both Con A- and CD2-induced proliferation were strongly inhibited by the addition of anti-CD25 MoAb. Furthermore, T-CLL lymphocytes acquired anti-tumour lytic activity after culture in the presence of PMA and ionomycin. We conclude that HTLV1- CD25- T-CLL can be characterized not only by morphological and phenotypical studies but also on the basis of signal requirements for cell activation.
为了确定白血病T细胞活化的信号需求，在给予不同刺激后，对一名HTLV-I阴性、CD4阳性、CD45RA阳性、CD45RO阳性、CD25阴性的T细胞型慢性淋巴细胞白血病患者外周淋巴细胞的增殖和白细胞介素-2（IL-2）产生情况进行了评估。与仅能通过双信号刺激激活的静息CD4阳性正常T淋巴细胞不同，T细胞型慢性淋巴细胞白血病细胞在没有辅助细胞（AC）和佛波酯肉豆蔻酸酯（PMA）的情况下，对一对抗CD2单克隆抗体（MoAbs）或刀豆蛋白A（Con A）产生增殖反应并释放IL-2。这两种刺激还能够在12至20小时内诱导大多数T细胞型慢性淋巴细胞白血病细胞表达CD25。仅在存在PMA的情况下检测到对抗CD3和抗CD28 MoAbs的反应，类似于在匹配表型的正常静息T淋巴细胞中观察到的情况。添加抗CD25 MoAb可强烈抑制Con A和CD2诱导的增殖。此外，T细胞型慢性淋巴细胞白血病淋巴细胞在PMA和离子霉素存在的情况下培养后获得了抗肿瘤溶解活性。我们得出结论，HTLV1阴性、CD25阴性的T细胞型慢性淋巴细胞白血病不仅可以通过形态学和表型研究来表征，还可以根据细胞活化的信号需求来表征。
