Uchiyama T, Kamio M, Kodaka T, Tamori S, Fukuhara S, Amakawa R, Uchino H, Araki K
First Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Blood. 1988 Oct;72(4):1182-6.
The proliferative response of fresh peripheral blood leukemic cells from eight adult T cell leukemia (ATL) patients to interleukin-4 (IL-4) was studied to determine the possibility that the IL-4-mediated T-cell growth pathway is involved in the cell growth of leukemic cells in ATL. Resting lymphocytes from ten normal individuals did not proliferate in response to IL-4. Leukemic cells from two ATL patients did not respond to interleukin-2 (IL-2) or IL-4. Leukemic cells from two patients did respond to IL-2, but not to IL-4. In contrast, a strong proliferative response was observed in the IL-4 culture, but not in the IL-2 culture in the remaining four patients. Chromosome analysis of mitotic cells, performed in one of four patients, confirmed that the cells dividing in response to IL-4 were leukemic cells, but not activated normal lymphocytes. These results indicate the activation of IL-4/IL-4 receptor system in leukemic cells from some ATL patients and suggest the possible involvement of the system in the proliferation of leukemic cells and the leukemogenesis in ATL.
研究了8例成人T细胞白血病（ATL）患者新鲜外周血白血病细胞对白介素-4（IL-4）的增殖反应，以确定IL-4介导的T细胞生长途径是否参与ATL白血病细胞的生长。10名正常个体的静息淋巴细胞对IL-4无增殖反应。2例ATL患者的白血病细胞对白介素-2（IL-2）或IL-4无反应。2例患者的白血病细胞对IL-2有反应，但对IL-4无反应。相反，其余4例患者在IL-4培养中观察到强烈的增殖反应，而在IL-2培养中未观察到。对4例患者中的1例进行有丝分裂细胞的染色体分析，证实对IL-4有反应而分裂的细胞是白血病细胞，而非活化的正常淋巴细胞。这些结果表明部分ATL患者白血病细胞中IL-4/IL-4受体系统被激活，并提示该系统可能参与白血病细胞的增殖及ATL的白血病发生过程。
