College of Life Science Shandong Agricultural University, Taian 271018, China.
Prostaglandins Other Lipid Mediat. 2009 Dec;90(3-4):94-7. doi: 10.1016/j.prostaglandins.2009.09.004. Epub 2009 Sep 16.
To explore the potential mechanism of how uterine innervations would affect the uterine mast cell (MC) population and functions during the periimplantation. We herein first examined the consequence of uterine neurectomy on embryo implantation events. We observed that amputation of autonomic nerves innervating the uterus led to on-time implantation failure in rats. Exploiting MC culture and ELISA approaches, we then further analyzed the effect of neurectomy on cellular histamine levels and its release from uterine MCs, to elucidate the relation of the autonomic nerves and local cellular immunity in the uterine during early pregnancy. We observed that disconnection of autonomic nerve innervation significantly increased the population of uterine MCs. Most interestingly, these increased number of uterine MCs in neuroectomized rats contained a much reduced cellular level of histamine. Our subsequent challenge experiments revealed that uterine MCs in nerve amputated rats exhibited enhanced histamine releasing rate in response to substance P and antiIgE, suggesting loss of nerve innervation in the uterus not only increases the population of uterine MCs, but also facilitates the release of histamine from MCs, thus subsequently interfere with the normal implantation process. Collectively, our findings provide a new line of evidence supporting the concept that immune-neuro-endocrine network plays important role during pregnancy establishment and maintenance.
为了探索在胚胎着床期间，子宫神经支配如何影响子宫肥大细胞（MC）群体和功能的潜在机制。我们首先检查了子宫神经切断术对胚胎着床事件的影响。我们观察到，自主神经支配子宫的切断导致大鼠按时着床失败。利用 MC 培养和 ELISA 方法，我们进一步分析了神经切断术对细胞组氨酸水平及其从子宫 MC 释放的影响，以阐明妊娠早期子宫中自主神经与局部细胞免疫的关系。我们观察到，自主神经支配的切断显著增加了子宫 MC 的数量。最有趣的是，在神经切断的大鼠中，这些增加的子宫 MC 中组氨酸的细胞水平明显降低。我们随后的挑战实验表明，神经切断大鼠的子宫 MC 在对 P 物质和抗 IgE 的反应中表现出增强的组氨酸释放率，这表明子宫神经支配的丧失不仅增加了子宫 MC 的数量，而且还促进了 MC 中组氨酸的释放，从而干扰了正常的着床过程。总的来说，我们的发现提供了新的证据，支持免疫-神经-内分泌网络在妊娠建立和维持过程中发挥重要作用的概念。
