Cocchiara R, Albeggiani G, Di Trapani G, Azzolina A, Lampiasi N, Rizzo F, Diotallevi L, Gianaroli L, Geraci D
Istituto di Biologia dello Sviluppo, CNR, Palermo, Italy.
Hum Reprod. 1992 Sep;7(8):1036-41. doi: 10.1093/oxfordjournals.humrep.a137790.
The relationship between maternal hormones and factors secreted by the implanting embryo is still controversial. We have analysed the in-vitro effect of oestradiol and human embryo-derived histamine-releasing factor (EHRF) on histamine release from rat uterine mast cells. Rat uterine mast cells which were preincubated with oestradiol and then challenged with human EHRF gave histamine release values two- to threefold higher than those without preincubation. The enhancement observed was time- and temperature-dependent. A similar enhancement was obtained with human sensitized basophils but not with rat peritoneal mast cells. Oestradiol, used as a direct challenge, did not induce any histamine release from either rat uterine or peritoneal mast cells, or from human sensitized basophils. Oestradiol preincubation also enhanced the histamine release induced by anti-IgE but did not enhance the histamine release induced by substance P or compound 48/80, two secretagogues that are not mediated by IgE. Moreover, uterine fragments derived from rats at various oestrus phases, with different amounts of endogenous oestrogen, were challenged in vitro with EHRF. The release of histamine by mast cells was higher at the proestrus and preimplantation phases than at dioestrus. All these findings suggest that the interaction of oestradiol with rat uterine mast cells was capable of enhancing in vitro the histamine releasing effect of EHRF.
母体激素与着床胚胎分泌的因子之间的关系仍存在争议。我们分析了雌二醇和人胚胎源性组胺释放因子（EHRF）对大鼠子宫肥大细胞组胺释放的体外作用。预先用雌二醇孵育然后用人EHRF刺激的大鼠子宫肥大细胞，其组胺释放值比未预先孵育的细胞高出两到三倍。观察到的增强作用是时间和温度依赖性的。用人致敏嗜碱性粒细胞也获得了类似的增强作用，但用大鼠腹膜肥大细胞则未观察到。用作直接刺激物的雌二醇，不会诱导大鼠子宫或腹膜肥大细胞或人致敏嗜碱性粒细胞释放任何组胺。雌二醇预孵育还增强了抗IgE诱导的组胺释放，但没有增强P物质或化合物48/80诱导的组胺释放，这两种促分泌剂不是由IgE介导的。此外，用EHRF对来自处于不同发情期且内源性雌激素含量不同的大鼠的子宫片段进行体外刺激。肥大细胞在动情前期和着床前期释放的组胺比在动情后期更高。所有这些发现表明，雌二醇与大鼠子宫肥大细胞的相互作用能够在体外增强EHRF的组胺释放效应。
