Cocchiara R, Albeggiani G, Di Trapani G, Azzolina A, Lampiasi N, Cervello G, Geraci D
Istituto di Biologia dello Sviluppo, CNR, Palermo, Italy.
J Reprod Immunol. 1988 Nov;14(2):191-201. doi: 10.1016/0165-0378(88)90069-1.
Rat uterine tissue was dissociated by enzymatic digestion with collagenase and viable mast cells were obtained. Their viability was assessed by the ability to exclude trypan blue dye and to respond functionally to different stimuli. Challenge with anti-IgE gave a calcium-dependent histamine release of 49%, whilst the undigested uterine fragments gave 23%. Moreover, they were capable of releasing histamine on challenge with the compound 48/80, suggesting a similarity with connective tissue mast cells. This similarity was further supported by their insensitivity to aldehyde blocking of dye binding. The final dispersed cell preparation contained 3 X 10(5) mast cells/g of uterine tissue, representing about 2% of total nucleated cells. The total histamine content of the undigested uterus was 2.5 micrograms/g of tissue, whilst after digestion the histamine determined was 1.2 pg per mast cell with a yield of 14%. The total histamine content of the uterus changed throughout the reproductive cycle, increasing before ovulation, reaching a maximum during ovulation and then decreasing after embryo implantation. This suggests that the implanting embryo, interacting with the uterus, may be capable of inducing the release of histamine. The embryo-derived histamine releasing factor (EHRF) that we have described previously is capable of inducing 22% histamine-release on uterine mast cells, thus supporting this hypothesis.
用胶原酶进行酶消化使大鼠子宫组织解离，从而获得活的肥大细胞。通过排除台盼蓝染料的能力以及对不同刺激的功能反应来评估它们的活力。用抗IgE刺激后，肥大细胞钙依赖性组胺释放率为49%，而未消化的子宫碎片组胺释放率为23%。此外，用化合物48/80刺激时它们能够释放组胺，这表明它们与结缔组织肥大细胞相似。它们对醛阻断染料结合不敏感，进一步支持了这种相似性。最终的分散细胞制剂每克子宫组织含有3×10⁵个肥大细胞，约占总核细胞的2%。未消化子宫的总组胺含量为每克组织2.5微克，而消化后测定每个肥大细胞的组胺含量为1.2皮克，产率为14%。子宫的总组胺含量在整个生殖周期中发生变化，在排卵前增加，排卵时达到最大值，然后在胚胎着床后下降。这表明着床的胚胎与子宫相互作用时，可能能够诱导组胺释放。我们之前描述的胚胎源性组胺释放因子（EHRF）能够诱导子宫肥大细胞释放22%的组胺，从而支持了这一假设。
