Shvemberger I N, Krylenkov V A, Stepan'ian L I, Sanchakova E V, Vakhtin Iu B
Tsitologiia. 1977 Jun;19(6):665-70.
The anterior chamber of mammalian eye (ACE) is one of the places of organism privileged immunologically that permits to use it for transplantations of allogenic tissues. Besides, the medium filling the chamber, provides an excellent growth of transplants there, whereas the transparent cornea allows to control the growth. The analysis of results obtained with this method shows its acceptibility both for heterotransplantations of tumors including human tumors, and for homotransplantations with the aim to study the progression of tumors and the selection of constant tumor lines. The ACE is easily applicable for studying morphogenetic potentialities of tumor cells, as well as their ability to differentiation and normalization, because its medium has the quality of "differentiating" for many tissues. Cytological heterogeneity, revealed during tumor transplantations, allows to study the spectrum of cytological variability and transgression of tumor characters at different stages of their progression. The ACE can be used for estimation of tumor formation ability of tumor cells by determining TD50 (tumor dosis 50) which is important for estimation of different kinds of damage (X-rays, chemotherapy etc.). According to authors' point of view, it is very perspective to use the anterior chamber of eye for elaboration of effective methods of hybridization tumor cells with normal ones.
哺乳动物眼的前房（ACE）是机体免疫特权部位之一，这使得它可用于同种异体组织移植。此外，填充前房的介质能使移植组织在其中良好生长，而透明角膜便于观察其生长情况。对该方法所得结果的分析表明，它不仅适用于包括人类肿瘤在内的肿瘤异种移植，也适用于旨在研究肿瘤进展和筛选稳定肿瘤细胞系的同种移植。ACE易于用于研究肿瘤细胞的形态发生潜能，以及它们的分化和正常化能力，因为其介质对许多组织具有“分化”特性。肿瘤移植过程中发现的细胞异质性，有助于研究肿瘤进展不同阶段的细胞变异谱和肿瘤特征的转变。通过测定TD50（肿瘤剂量50），ACE可用于评估肿瘤细胞的成瘤能力，这对评估不同类型的损伤（如X射线、化疗等）很重要。作者认为，利用眼的前房来研发肿瘤细胞与正常细胞有效杂交的方法极具前景。
