Helmholtz Center for Infection Research, Chemical Microbiology, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Letiya Octyabrya 7, korp. 2, Moscow 117811, Russia.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1837-1843. doi: 10.1099/ijs.0.016832-0. Epub 2009 Sep 18.
Eight strains of Gram-negative, bacteroid-shaped, prosthecate bacteria, isolated from brackish water (MCS24T, MCS17 and MCS35), the marine environment (CM260, CM272 and CM282) and activated sludge (FWC40T and FWC43T), were characterized using a polyphasic approach. Analysis of 16S rRNA gene sequences determined that all strains were affiliated to the alphaproteobacterial genus Brevundimonas, forming three distinct phyletic lineages within the genus. The strains grew best with 5-30 g NaCl l(-1) at 20-30 degrees C. DNA G+C contents for strains MCS24T, FWC40T and FWC43T were between 65 and 67 mol%, in accordance with values reported previously for other species of the genus. Moreover, chemotaxonomic data and physiological and biochemical tests allowed the phenotypic differentiation of three novel species within the genus Brevundimonas, for which the names Brevundimonas halotolerans sp. nov. (type strain MCS24T =LMG 25346T =CCUG 58273T), Brevundimonas poindexterae sp. nov. (type strain FWC40T =LMG 25261T =CCUG 57883T) and Brevundimonas staleyi sp. nov. (type strain FWC43T =LMG 25262T =CCUG 57884T) are proposed.
从咸水（MCS24T、MCS17 和 MCS35）、海洋环境（CM260、CM272 和 CM282）和活性污泥（FWC40T 和 FWC43T）中分离到 8 株革兰氏阴性、拟杆菌状、有菌毛的细菌，采用多相分类法对其进行了表征。16S rRNA 基因序列分析确定，所有菌株均属于 α-变形菌纲的短杆菌属，在属内形成三个不同的系统发育谱系。这些菌株在 20-30°C、5-30g NaCl/L 的条件下生长最好。菌株 MCS24T、FWC40T 和 FWC43T 的 DNA G+C 含量在 65-67mol%之间，与该属其他种的报道值相符。此外，化学分类数据和生理生化试验允许对短杆菌属内的三个新种进行表型区分，为此提议了短杆菌属耐盐菌（Brevundimonas halotolerans sp. nov.，模式菌株 MCS24T=LMG 25346T=CCUG 58273T）、短杆菌属波因德克斯特菌（Brevundimonas poindexterae sp. nov.，模式菌株 FWC40T=LMG 25261T=CCUG 57883T）和短杆菌属斯泰利氏菌（Brevundimonas staleyi sp. nov.，模式菌株 FWC43T=LMG 25262T=CCUG 57884T）。
