Division of Pathogen Resource Management, National Institute of Infectious Diseases, National Institute of Health, Korea Disease Control and Prevention Agency (KDCA), Cheongju, 28160, Republic of Korea.
Antonie Van Leeuwenhoek. 2024 Sep 26;118(1):11. doi: 10.1007/s10482-024-02020-5.
An aerobic, Gram-stain-negative, non-motile, non-spore-forming, short rod-shaped bacterial strain, designated NCCP 15609 , was isolated from the blood sample of a patient in the Republic of Korea. The strain was identified as Brevundimonas diminuta using MALDI-TOF. A phylogenetic tree constructed using 16S rRNA gene sequences revealed that the isolate was of the genus Brevundimonas with 99.8% similarity to B. naejangsanensis. The strain NCCP 15609 genome consisted of one contig with 3,063,090 bp, and had a G+C content of 67.4%. The genome contained 2,949 protein-coding sequences, 52 tRNAs, and 6 rRNAs. The DNA-DNA hybridisation between NCCP 15609 and B. naejangsanensis yielded 92.5% and 49.5% ± 2.6%, respectively, using the average nucleotide identity (ANI) and digital DNA-DNA hybridisation (dDDH). The predominant fatty acids of strain NCCP 15609 were summed feature 8 (C ω7c/C ω6c) and C. The isolate contained polar lipids and quinone, corresponding to phosphatidylglycerol, 1,2-di-O-acyl-3-O-[D-glycopyranosyl (1 → 4)-α-D-glucopyranuronosyl] glycerol, and ubiquinone-10, respectively. Based on its phylogenetic, physiological, and chemotaxonomic characteristics, we suggest that NCCP 15609 represents a novel pathogen resource of the genus Brevundimonas and propose to name it Brevundimonas sanguinis sp. nov. The type strain is NCCP 15609 (= DSM 116005).
一株需氧、革兰氏阴性、非运动、非孢子形成、短杆状细菌菌株，被命名为 NCCP 15609，从韩国一名患者的血液样本中分离得到。该菌株通过 MALDI-TOF 鉴定为苍白杆菌属的微弱苍白杆菌。基于 16S rRNA 基因序列构建的系统发育树显示，该分离株属于苍白杆菌属，与 Naejangsanensis 苍白杆菌的相似度为 99.8%。菌株 NCCP 15609 基因组由一个含有 3,063,090 bp 的连续体组成，GC 含量为 67.4%。基因组包含 2,949 个蛋白编码序列、52 个 tRNA 和 6 个 rRNA。NCCP 15609 与 Naejangsanensis 之间的 DNA-DNA 杂交率分别为 92.5%和 49.5%±2.6%，使用平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）计算。菌株 NCCP 15609 的主要脂肪酸为特征脂肪酸 8（C ω7c/C ω6c）和 C 12:0 。该分离株含有极性脂类和醌类，分别对应于磷脂酰甘油、1,2-二酰基-3-O-[D-吡喃糖基（1→4）-α-D-吡喃葡萄糖基]甘油和泛醌-10。基于其系统发育、生理和化学分类特征，我们建议 NCCP 15609 代表苍白杆菌属的一种新型病原体资源，并提议将其命名为微弱血杆菌 Brevundimonas sanguinis sp. nov.。模式菌株为 NCCP 15609（=DSM 116005）。
