Kalins' ka L M, Mestechkina A Ia
Ukr Biokhim Zh. 1977 Jul-Aug;49(4):92-6.
Adrenalectomy and administration of hydrocortisone and ACTH are shown to induce no changes in the total activity of neutral peptide-hydrolase in both homogenates and brain subcellular fractions. The absence of adrenalectomy and hormone administration effect on the total peptide-hydrolase activity in homogenates is established simultaneously with its essential changes in the brain subcellular fractions. A decrease in the enzymic activity of the mitochondrial-lysosomal fraction (MLF) following adrenalectomy is observed side by side with its increase in a soluble fraction. Hydrocortisone and ACTH administration cause an increase in the acid peptide-hydrolase activity in MLF and its decrease in the brain soluble and microsomal fractions of adrenalectomized rabbits. The degree of solubilization of MLF acid and neutral peptide-hydrolase by detergent triton X-100 after adrenalectomy is increased. Hydrocortisone and ACTH administration, on the contrary, decrease a degree of solubilization of the brain MLF peptide-hydrolase in adrenalectomized animals.
肾上腺切除术以及氢化可的松和促肾上腺皮质激素的给药均未导致匀浆和脑亚细胞组分中中性肽水解酶的总活性发生变化。在确定肾上腺切除术和激素给药对匀浆中总肽水解酶活性无影响的同时，发现其在脑亚细胞组分中发生了显著变化。肾上腺切除术后，线粒体 - 溶酶体组分（MLF）的酶活性降低，而可溶组分中的酶活性增加。给肾上腺切除的兔子注射氢化可的松和促肾上腺皮质激素会导致MLF中酸性肽水解酶活性增加，而在脑可溶组分和微粒体组分中活性降低。肾上腺切除术后，去污剂曲拉通X - 100对MLF酸性和中性肽水解酶的增溶程度增加。相反，给肾上腺切除的动物注射氢化可的松和促肾上腺皮质激素会降低脑MLF肽水解酶的增溶程度。
