Cvejić Jelena, Malencić Dorde, Tepavcević Vesna, Posa Mihalj, Miladinović Jegor
Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia.
Nat Prod Commun. 2009 Aug;4(8):1069-74.
The growing clinical interest and use of soybean-based food products and extracts to increase dietary phytoestrogen intake have led to medical interest in the precise determination of the phytoestrogen composition of soybean products. We have analyzed the composition of these compounds in 20 domestic and introduced varieties of genetically non-modified soybean genotypes grown under the same agroecological conditions. The isoflavone content of soybeans cultivated in this region of Serbia has not been previously reported. The assays were performed, after extraction with methanol-water (8:2, v/v), by C18 reversed phase high-performance liquid chromatography coupled with photodiode array detection. The total phytoestrogen concentration was found to be between 2.24 and 3.79 mg g(-1) dry bean weight. The total concentration of daidzein and its derivatives ranged from 0.96 to 1.82 mg g(-1), total glyciteines from 0.34-0.53 mg g(-1), and all genistein derivatives from 0.86-1.67 mg g(-1) dry bean weight. Given the high biological potency of phytoestrogens and their metabolic conversion products, our data suggest that phytoestrogen content screening could be a useful tool in the selection of soybean genotypes with improved health promoting characteristics.
对基于大豆的食品和提取物的临床兴趣与日俱增，人们希望通过食用这些产品来增加膳食中植物雌激素的摄入量，这引发了医学界对精确测定大豆产品中植物雌激素成分的关注。我们分析了在相同农业生态条件下种植的20个国内和引进的非转基因大豆基因型品种中这些化合物的成分。此前尚未有关于塞尔维亚该地区种植大豆异黄酮含量的报道。在用甲醇 - 水（8:2，v/v）提取后，通过C18反相高效液相色谱结合光电二极管阵列检测进行分析。发现总植物雌激素浓度在2.24至3.79毫克/克（干豆重量）之间。大豆苷元及其衍生物的总浓度范围为0.96至1.82毫克/克，总染料木黄酮为0.34 - 0.53毫克/克，所有染料木素衍生物为0.86 - 1.67毫克/克（干豆重量）。鉴于植物雌激素及其代谢转化产物具有很高的生物活性，我们的数据表明，植物雌激素含量筛选可能是选择具有改善健康促进特性的大豆基因型的有用工具。
