Kiselev F L, Sadogurskaia E M, Gorshunova L P
Vopr Virusol. 1977 Mar-Apr(2):184-9.
In the present study we attempted to follow the fate of the parental poliomyelitis virus of immunological type II labeled with 3H-uridine in vaccinated animals. For this purpose, 3H-uridine labeled purified and concentrated virus was inoculated intramuscularly into cotton rats (sensitive to poliomyelitis virus) and guinea pigs (insensitive to poliomyelitis virus). The controls consisted of two groups of the same animal species: the animals of the first group were inoculated intramuscularly with pure 3H-uridine of known high activity while the animals of the second group were inoculated also intramuscularly with pure 3H-uridine and in another leg 0.5 ml of standard poliovirus vaccine of type II. Since poliomyelitis virus replicates in the cytoplasm, we studied cytoplasmic extracts of various organs (the brain, spinal cord, lungs, and spleen) of cotton rats and guinea pigs at different intervals after virus inoculation (1, 4, and 15 days). The experimental results showed the attenuated type II poliomyelitis virus to be able to persist for long periods in animals in the form of subviral particles with a higher buoyant density in cesium chloride gradient.
在本研究中，我们试图追踪经3H-尿苷标记的免疫II型脊髓灰质炎亲代病毒在接种动物体内的去向。为此，将经3H-尿苷标记的纯化浓缩病毒肌肉注射到棉鼠（对脊髓灰质炎病毒敏感）和豚鼠（对脊髓灰质炎病毒不敏感）体内。对照组由两组相同动物组成：第一组动物肌肉注射已知高活性的纯3H-尿苷，而第二组动物也肌肉注射纯3H-尿苷，并在另一条腿上注射0.5 ml II型标准脊髓灰质炎疫苗。由于脊髓灰质炎病毒在细胞质中复制，我们在接种病毒后的不同时间间隔（1天、4天和15天）研究了棉鼠和豚鼠各种器官（脑、脊髓、肺和脾）的细胞质提取物。实验结果表明，减毒II型脊髓灰质炎病毒能够以在氯化铯梯度中具有较高浮力密度的亚病毒颗粒形式在动物体内长期存在。
