National Physical Laboratory, Hampton Road, Teddington, Middlesex TW11 0LW, United Kingdom.
Colloids Surf B Biointerfaces. 2010 Jan 1;75(1):275-81. doi: 10.1016/j.colsurfb.2009.08.049. Epub 2009 Sep 6.
This paper describes the use of nanoparticle characterisation tools to evaluate the interaction between bovine serum albumin (BSA) and dispersed nanoparticles in aqueous media. Dynamic light scattering, zeta-potential measurements and scanning electron microscopy were used to probe the state of zinc oxide (ZnO) and titanium dioxide (TiO(2)) nanoparticles in the presence of various concentrations of BSA, throughout a three-day period. BSA was shown to adhere to ZnO but not to TiO(2). The adsorption of BSA led to subsequent de-agglomeration of the sub-micron ZnO clusters into smaller fragments, even breaking them up into individual isolated nanoparticles. We propose that certain factors, such as adsorption kinetics of BSA on to the surface of ZnO, as well as the initial agglomerated state of the ZnO, prior to BSA addition, are responsible for promoting the de-agglomeration process. Hence, in the case of TiO(2) we see no de-agglomeration because: (a) the nanoparticles are more highly agglomerated to begin with and (b) BSA does not adsorb effectively on the surface of the nanoparticles. The zeta-potential results show that, for either ZnO or TiO(2), the presence of BSA resulted in enhanced stability. In the case of ZnO, the enhanced stability is limited to BSA concentrations below 0.5 wt.%. Steric and electrostatic repulsion are thought to be responsible for improved stability of the dispersion.
本文使用纳米颗粒特性分析工具研究牛血清白蛋白（BSA）与分散在水介质中的纳米颗粒之间的相互作用。通过动态光散射、动电位测量和扫描电子显微镜研究了在不同浓度 BSA 存在的情况下，氧化锌（ZnO）和二氧化钛（TiO(2)）纳米颗粒在三天内的状态。结果表明，BSA 与 ZnO 结合，但不与 TiO(2)结合。BSA 的吸附导致亚微米 ZnO 簇随后解团聚成更小的碎片，甚至将它们分解成单个孤立的纳米颗粒。我们提出，某些因素，如 BSA 在 ZnO 表面的吸附动力学以及 BSA 加入之前 ZnO 的初始团聚状态，是促进解团聚过程的原因。因此，在 TiO(2)的情况下，我们没有看到解团聚，因为：(a) 纳米颗粒一开始就高度团聚，(b) BSA 不能有效地吸附在纳米颗粒表面。动电位结果表明，对于 ZnO 或 TiO(2)，BSA 的存在导致分散体的稳定性增强。在 ZnO 的情况下，稳定性的增强仅限于 BSA 浓度低于 0.5wt%。认为空间和静电排斥是分散体稳定性提高的原因。
