Kiyatkin N I, Dulubova I E, Chekhovskaya I A, Grishin E V
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
FEBS Lett. 1990 Sep 17;270(1-2):127-31. doi: 10.1016/0014-5793(90)81250-r.
cDNA encoding the putative alpha-latrotoxin precursor was isolated from spider venom glands cDNA library and sequenced. The cDNA contained the 4203 base-pair open reading frame corresponding to the 156,855-Da protein composed of 1401 amino acids. Computer analysis of the deduced primary structure revealed the presence of various internal imperfect repeats mainly in its central and C-terminal regions.
从蜘蛛毒腺cDNA文库中分离出编码假定α-拉托毒素前体的cDNA并进行测序。该cDNA包含4203个碱基对的开放阅读框，对应于由1401个氨基酸组成的156,855道尔顿的蛋白质。对推导的一级结构进行计算机分析发现，主要在其中心区域和C端区域存在各种内部不完全重复序列。
