McLean M, Gerats A G, Baird W V, Meagher R B
Department of Genetics, University of Georgia, Athens 30602.
J Hered. 1990 Sep-Oct;81(5):341-6.
The Mitchell variety of Petunia hybrida possesses a superfamily of actin genes which contains between 100 and 200 members that can be divided into at least six highly divergent subfamilies. The segregation of restriction fragment length polymorphisms among 96 plants from two backcrosses between the Violet 23 and Red 51 Petunia varieties and the Violet 23 x Red 51 hybrid was examined using gene-specific probes from six Petunia actin gene subfamilies. These data were compared with the genotypes of each plant at 11 marker loci which are distributed among the seven chromosomes of Petunia and which determine flower, pollen, and isozyme phenotypes. From these analyses, members of these six actin gene subfamilies were mapped to five locations on five Petunia chromosomes: the PAc9, PAc1, PAc4, and PAc2 subfamilies are on chromosomes I, II, III, and VII respectively; the PAc3 and PAc7 subfamilies are tightly linked on chromosome IV. All members of the PAc4 subfamily cosegregated as a cluster of genes. These data are discussed regarding gene amplification in plants.
矮牵牛的米切尔变种拥有一个肌动蛋白基因超家族，其中包含100到200个成员，这些成员可分为至少六个高度不同的亚家族。使用来自六个矮牵牛肌动蛋白基因亚家族的基因特异性探针，检测了紫23和红51矮牵牛品种以及紫23×红51杂种之间两个回交产生的96株植株中限制性片段长度多态性的分离情况。将这些数据与每株植物在11个标记位点的基因型进行比较，这些标记位点分布在矮牵牛的七条染色体上，决定花、花粉和同工酶表型。通过这些分析，这六个肌动蛋白基因亚家族的成员被定位到矮牵牛五条染色体的五个位置上：PAc9、PAc1、PAc4和PAc2亚家族分别位于第一、二、三、七条染色体上；PAc3和PAc7亚家族紧密连锁在第四条染色体上。PAc4亚家族的所有成员作为一个基因簇共同分离。文中针对植物中的基因扩增对这些数据进行了讨论。
