Orskov F, Whittam T S, Cravioto A, Orskov I
International Escherichia and Klebsiella Centre, Statens Seruminstitut, Copenhagen, Denmark.
J Infect Dis. 1990 Jul;162(1):76-81. doi: 10.1093/infdis/162.1.76.
Fifty Escherichia coli strains belonging to nine classic EPEC (enteropathogenic E. coli) O:H serotypes from infantile diarrhea were examined for enzyme polymorphism and typed according to their multilocus genotypes. They were further examined for lipopolysaccharide (LPS) patterns by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by silver staining, for localized adhesion to HEp2 cells, and for their outer membrane protein (OMP) pattern. A very close relationship was detected among electrophoretic type, O:H serotype, biotype, LPS type, and OMP pattern. Most likely these characteristic EPEC groups (all type I) each represented bacterial clones, and furthermore they could be grouped into two genetically closely related clusters. In each cluster the only phenotypic character that differed among strains was the O antigen, which might suggest that the genetic background for the LPS side chains can be transferred horizontally among EPEC strains. The results support the idea that O:H serotyping is useful for studies of epidemiologic linkages between cases of EPEC diarrhea.
对来自婴儿腹泻的50株属于9种经典肠致病性大肠杆菌（EPEC）O:H血清型的大肠杆菌菌株进行了酶多态性检测，并根据其多位点基因型进行分型。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后银染检测其脂多糖（LPS）模式，检测其对HEp2细胞的局部黏附情况，并检测其外膜蛋白（OMP）模式。在电泳型、O:H血清型、生物型、LPS型和OMP模式之间检测到非常密切的关系。很可能这些特征性的EPEC组（均为I型）各自代表细菌克隆，此外它们可分为两个遗传关系密切的簇。在每个簇中，菌株间唯一不同的表型特征是O抗原，这可能表明LPS侧链的遗传背景可在EPEC菌株间水平转移。结果支持O:H血清分型对研究EPEC腹泻病例间的流行病学联系有用这一观点。
