R&D, Ash Access Technology, Inc., 3601 Sagamore Parkway North, Lafayette, IN 47904, USA.
J Infect. 2010 Jan;60(1):36-43. doi: 10.1016/j.jinf.2009.09.013. Epub 2009 Sep 22.
The objective of this study was to evaluate the bactericidal activity of a new antiseptic in an in vitro model using reference bacterial strains and on abdominal and inguinal skin of healthy human subjects. ZuraPrep (C/MB/P/IPA) contains citrate, methylene blue, parabens and isopropyl alcohol 70%.
In vitro and in vivo studies evaluate immediate and persistent activity of the tested solution using bovine rawhides inoculated with single strains of microorganisms and natural bacterial flora on skin of adult human subjects. Depending upon the anatomical site (abdominal or inguinal), several different configurations of test times were executed. Post-prep surface cultures were performed at 10 min and 6h after application of the test solution.
Tested solution showed high efficiency in log reduction of viable microbes both in vitro and in vivo. Post-prep activity 10 min after application ranged from 2.5 to 3 log(10) reduction from baseline on abdominal sites and 3.5-4.5 on inguinal sites. Similar levels of reduction persisted 6h after application.
The level of antisepsis provided by the tested solution is similar or greater than that obtained with other antiseptics currently in use, and further clinical testing of the new antiseptic is warranted.
本研究旨在通过参考细菌株和健康人体腹部及腹股沟皮肤的体外模型评估一种新型防腐剂的杀菌活性。ZuraPrep（C/MB/P/IPA）含有柠檬酸盐、亚甲蓝、对羟基苯甲酸酯和 70%异丙醇。
体外和体内研究使用牛生皮接种单一微生物菌株和人体皮肤的天然细菌菌群来评估测试溶液的即时和持久活性。根据解剖部位（腹部或腹股沟），执行了几种不同的测试时间配置。在应用测试溶液后 10 分钟和 6 小时进行预处理后表面培养。
测试溶液在体外和体内均显示出对活微生物的高效对数减少。预处理后 10 分钟，腹部部位的活性从基线降低了 2.5 到 3 个对数（10），腹股沟部位降低了 3.5 到 4.5。应用后 6 小时仍保持类似水平的减少。
测试溶液提供的消毒水平与目前使用的其他防腐剂相似或更高，需要进一步对新型防腐剂进行临床测试。
