用于血清学鉴别HTLV-I和HTLV-II感染的型特异性抗原。
Type-specific antigens for serological discrimination of HTLV-I and HTLV-II infection.
作者信息
Chen Y M, Lee T H, Wiktor S Z, Shaw G M, Murphy E L, Blattner W A, Essex M
机构信息
Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115.
出版信息
Lancet. 1990 Nov 10;336(8724):1153-5. doi: 10.1016/0140-6736(90)92769-e.
55 HTLV-I (human T-cell lymphotropic virus) and 45 HTLV-II carriers, confirmed by HTLV-type specific polymerase chain reaction (PCR), were distinguished by western blot assays with recombinant HTLV I or II envelope glycoproteins. Recombinant protein (RP) B1 contains aminoacids 166-201 from HTLV-I exterior glycoprotein gp46 and was reactive with HTLV-I samples only. RP-IIB, which contains aminoacids 96-235 from HTLV-II exterior glycoprotein gp52, was reactive with all HTLV-II samples. 39 patients (86.6%) had high reactivity by densitometry. Of 55 HTLV-I samples, 35 (65.5%) had antibody reactivity to RP-IIB, but only 1 (1.8%) had high reactivity by densitometry. RP B1 and IIB western blot assays may replace the PCR test in diagnosis of HTLV infection.
通过HTLV型特异性聚合酶链反应（PCR）确认的55例HTLV-I（人类T细胞嗜淋巴细胞病毒）携带者和45例HTLV-II携带者，采用重组HTLV I或II包膜糖蛋白的蛋白质印迹分析进行区分。重组蛋白（RP）B1包含来自HTLV-I外部糖蛋白gp46的166 - 201位氨基酸，仅与HTLV-I样本反应。RP-IIB包含来自HTLV-II外部糖蛋白gp52的96 - 235位氨基酸，与所有HTLV-II样本反应。39例患者（86.6%）通过密度测定法具有高反应性。在55例HTLV-I样本中，35例（65.5%）对RP-IIB有抗体反应，但通过密度测定法只有1例（1.8%）具有高反应性。RP B1和IIB蛋白质印迹分析在HTLV感染诊断中可能取代PCR检测。
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