用免疫荧光法检测和区分抗人I型和II型嗜T细胞病毒抗体。
Detection and differentiation of antibodies to human T-cell lymphotropic virus types I and II by the immunofluorescence method.
作者信息
Gallo D, Penning L M, Hanson C V
机构信息
Viral and Rickettsial Disease Laboratory, State of California Department of Health Services, Berkeley 94704.
出版信息
J Clin Microbiol. 1991 Oct;29(10):2345-7. doi: 10.1128/jcm.29.10.2345-2347.1991.
Abstract
We compared the sensitivities of the prototype human T-cell lymphotropic virus type I (HTLV-I)- and HTLV-II-transformed cell lines, MT2 and Mo-T, with that of an HTLV-II-infected cell line, clone 19, established in our laboratory, in the immunofluorescence (IF) test for detection of antibody to HTLV-I and HTLV-II. In addition, IF antibody titers with the three antigens were determined, and the results were compared with HTLV-I and HTLV-II typing by polymerase chain reaction (PCR). The MT2 cell line was more sensitive than the two HTLV-II cell lines for detecting HTLV-I antibody by IF, and clone 19 was more sensitive than Mo-T or MT2 for measuring HTLV-II antibody. In the titration study, the antigen that gave the highest titer correlated completely with the HTLV type determined by PCR, indicating that the relatively simple IF titration method can be used for differentiating HTLV-I and HTLV-II antibody in sera and plasmas.
摘要
我们在检测抗人嗜T淋巴细胞病毒I型（HTLV-I）和HTLV-II抗体的免疫荧光（IF）试验中，比较了原型HTLV-I和HTLV-II转化细胞系MT2和Mo-T与我们实验室建立的一株HTLV-II感染细胞系克隆19的敏感性。此外，还测定了这三种抗原的IF抗体滴度，并将结果与聚合酶链反应（PCR）进行的HTLV-I和HTLV-II分型结果进行比较。MT2细胞系在通过IF检测HTLV-I抗体方面比两个HTLV-II细胞系更敏感，而克隆19在检测HTLV-II抗体方面比Mo-T或MT2更敏感。在滴定研究中，产生最高滴度的抗原与通过PCR确定的HTLV类型完全相关，这表明相对简单的IF滴定法可用于区分血清和血浆中的HTLV-I和HTLV-II抗体。
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