Bogiel Tomasz, Mikucka Agnieszka, Deptuła Aleksander, Gospodarek Eugenia
Katedra i Zaklad Mikrobiologii Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy Uniwersytet Mikołaja Kopernika w Toruniu.
Med Dosw Mikrobiol. 2009;61(2):111-8.
Many identification and relatedness studies methods had been commonly used for epidemiological studies in microbiological laboratories. Apart from phenotypic methods, genotypic are also often used. The aim of this study was to compare, obtained by PFGE chromosomal DNA patterns of methicillin-resistant S. epidermidis strains isolated from clinical material. 46 methicillin-resistant S. epidermidis strains were included in this study. Most of them were isolated from wound swabs (65.2%) and catheters (19.6%) from different surgical clinics (76.1%). To identify strains and receive biochemical profiles, ID 32 Staph tests and GPI cards of Vitek 1 system were used. Pulsed-field gel electrophoresis and Tenover et al. interpretation were used to compare chromosomal DNA patterns of examined strains. 44 and 42 PFGE patterns of chromosomal DNA were received, using visual interpretation classifying two pairs of strains as the same, two pairs as closely related and three pairs as probably related. Strains classified as identical and similar in visual evaluation were indistinguishable in Molecular Analyst DST interpretation, probably due to tolerance in bands location pattern. Strains probably related in visual interpretation represent at least 96% similarity in Molecular Analyst DST but different susceptibility and biochemical profiles obtained by ID 32 Staph and Vitek 1. PFGE analysis had foremost capacity to distinguish methicillin-resistant S. epidermidis strains using visual interpretation and Molecular Analyst DST (Bio-Rad) program and seems to be useful method in epidemiological studies. Strains with the same PFGE pattern, had different susceptibility and biochemical profiles.
许多鉴定及亲缘关系研究方法已普遍用于微生物实验室的流行病学研究。除了表型方法外，基因型方法也经常被使用。本研究的目的是比较从临床材料中分离出的耐甲氧西林表皮葡萄球菌菌株通过脉冲场凝胶电泳（PFGE）获得的染色体DNA图谱。本研究纳入了46株耐甲氧西林表皮葡萄球菌菌株。其中大多数菌株是从不同外科诊所（76.1%）的伤口拭子（65.2%）和导管（19.6%）中分离出来的。为了鉴定菌株并获得生化图谱，使用了Vitek 1系统的ID 32 Staph测试和GPI卡。采用脉冲场凝胶电泳和Tenover等人的解释方法来比较所检测菌株的染色体DNA图谱。通过视觉判读获得了44种和42种染色体DNA的PFGE图谱，将两对菌株分类为相同，两对为密切相关，三对为可能相关。在视觉评估中分类为相同和相似的菌株在Molecular Analyst DST判读中无法区分，这可能是由于条带位置模式的耐受性。在视觉判读中可能相关的菌株在Molecular Analyst DST中显示至少96%的相似性，但通过ID 32 Staph和Vitek 1获得的药敏性和生化图谱不同。PFGE分析在使用视觉判读和Molecular Analyst DST（Bio-Rad）程序区分耐甲氧西林表皮葡萄球菌菌株方面具有首要能力，似乎是流行病学研究中的一种有用方法。具有相同PFGE图谱的菌株具有不同的药敏性和生化图谱。
