Longa C M O, Pertot I
IASMA Research and Innovation Center, Fondazione Edmund Mach, 38010 S. Michele all'Adige (TN), Italy.
Lett Appl Microbiol. 2009 Nov;49(5):609-14. doi: 10.1111/j.1472-765X.2009.02715.x. Epub 2009 Aug 18.
To develop an intact soil-core microcosm method to study the survival and vertical dispersal of an experimental biocontrol agent (Trichoderma atroviride SC1) applied to the soil surface.
The soil for the microcosms was collected using iron pipes with perforations corresponding to different soil layers. The tool was inserted into the soil and gently removed with the soil core inside. Trichoderma atroviride SC1 was mixed with the top layer of soil in the pipe. The experiment was performed in 2006 and 2007, and data from the microcosms were compared with results obtained under field conditions in the locations in which, the microcosms were collected, in the same periods. The concentrations of T. atroviride SC1 in the soil were estimated immediately after treatment, and 1, 5, 9 and 18 weeks after treatment at both the soil surface and the above-mentioned depths. The development of T. atroviride SC1 populations in the microcosms during the 18 weeks of monitoring was similar to that observed under field conditions. The dispersal of conidia was affected by the application of water to the soil.
Results demonstrate that this microcosm prototype can be used to model the behaviour of T. atroviride SC1 in soil.
The intact soil-core microcosm is a reliable, easy-to-use, fast and cheap method that could also be used in studies of similar filamentous fungi to study their probable fate in the soil prior to their being introduced into the environment.
开发一种完整土芯微观模型方法，以研究施用于土壤表面的实验性生物防治剂（深绿木霉SC1）的存活及垂直扩散情况。
使用带有对应不同土壤层穿孔的铁管收集用于微观模型的土壤。将该工具插入土壤，然后连同内部的土芯轻轻取出。把深绿木霉SC1与管内表层土壤混合。实验于2006年和2007年进行，并将微观模型的数据与同期在收集微观模型土壤的地点的田间条件下获得的结果进行比较。在处理后立即以及处理后1、5、9和18周，对土壤表面及上述深度处的深绿木霉SC1浓度进行估算。在18周的监测期内，微观模型中深绿木霉SC1种群的发展情况与田间条件下观察到的相似。分生孢子的扩散受到向土壤浇水的影响。
结果表明，这种微观模型原型可用于模拟深绿木霉SC1在土壤中的行为。
完整土芯微观模型是一种可靠、易用、快速且廉价的方法，也可用于类似丝状真菌的研究，以在将其引入环境之前研究它们在土壤中的可能命运。
