SafeCrop Centre, Fondazione Edmund Mach, San Michele all'Adige, Trento, Italy.
J Appl Microbiol. 2009 May;106(5):1549-57. doi: 10.1111/j.1365-2672.2008.04117.x. Epub 2009 Feb 4.
To study the survival in the soil and the dispersion in the environment of Trichoderma atroviride SC1 after soil applications in a vineyard.
Trichoderma atroviride SC1 was introduced into soil in two consecutive years. The levels of T. atroviride populations at different spatial and temporal points following inoculation were assessed by counting the colony-forming units and by a specific quantitative real-time PCR. A high concentration of T. atroviride SC1 was still observed at the 18th week after inoculation. The vertical migration of the fungus to a soil depth of 0.4 m was already noticeable during the first week after inoculation. The fungus spread up to 4 m (horizontally) from the point of inoculation and its concentration decreased with the increasing distance (horizontal and vertical). It was able to colonize the rhizosphere and was also found on grapevine leaves. One year after soil inoculation, T. atroviride SC1 could still be recovered in the treated areas.
Trichoderma atroviride SC1 survived and dispersed becoming an integrant part of the local microbial community under the tested conditions.
The persistence and rapid spread of T. atroviride SC1 represent good qualities for its future use as biocontrol agent against soilborne pathogens.
研究木霉 SC1 在葡萄园土壤中的生存和在环境中的扩散。
木霉 SC1 在连续两年被引入土壤。通过计数菌落形成单位和特定的定量实时 PCR 来评估接种后不同时空点的木霉种群水平。在接种后第 18 周仍观察到高浓度的木霉 SC1。在接种后的第一周，真菌已经向土壤深度 0.4 米的垂直迁移。真菌从接种点水平传播到 4 米（水平），其浓度随距离（水平和垂直）的增加而降低。它能够定殖根际，也在葡萄叶片上发现。土壤接种一年后，仍可在处理区回收木霉 SC1。
在测试条件下，木霉 SC1 存活并扩散，成为当地微生物群落的组成部分。
木霉 SC1 的持久性和快速传播是其作为防治土传病原菌的生物防治剂的未来应用的良好品质。
