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组织蛋白酶L是NIH3T3细胞中嗜亲性鼠白血病病毒感染所必需的。

Cathepsin L is required for ecotropic murine leukemia virus infection in NIH3T3 cells.

作者信息

Yoshii Hiroaki, Kamiyama Haruka, Minematsu Kazuo, Goto Kensuke, Mizota Tsutomu, Oishi Kazunori, Katunuma Nobuhiko, Yamamoto Naoki, Kubo Yoshinao

机构信息

Department of AIDS Research, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.

出版信息

Virology. 2009 Nov 25;394(2):227-34. doi: 10.1016/j.virol.2009.08.045. Epub 2009 Sep 24.

DOI:10.1016/j.virol.2009.08.045
PMID:19781728
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7111982/
Abstract

Recently it has been reported that a cathepsin B inhibitor, CA-074Me, attenuates ecotropic murine leukemia virus (Eco-MLV) infection in NIH3T3 cells, suggesting that cathepsin B is required for the Eco-MLV infection. However, cathepsin B activity was negative or extremely low in NIH3T3 cells. How did CA-074Me attenuate the Eco-MLV infection? The CA-074Me treatment of NIH3T3 cells inhibited cathepsin L activity, and a cathepsin L specific inhibitor, CLIK148, attenuated the Eco-MLV vector infection. These results indicate that the suppression of cathepsin L activity by CA-074Me induces the inhibition of Eco-MLV infection, suggesting that cathepsin L is required for the Eco-MLV infection in NIH3T3 cells. The CA-074Me treatment inhibited the Eco-MLV infection in human cells expressing the exogenous mouse ecotropic receptor and endogenous cathepsins B and L, but the CLIK148 treatment did not, showing that only the cathepsin L suppression by CLIK148 is not enough to prevent the Eco-MLV infection in cells expressing both of cathepsins B and L, and CA-074Me inhibits the Eco-MLV infection by suppressing both of cathepsins B and L. These results suggest that either cathepsin B or L is sufficient for the Eco-MLV infection.

摘要

最近有报道称，组织蛋白酶B抑制剂CA - 074Me可减弱嗜亲性鼠白血病病毒（Eco - MLV）在NIH3T3细胞中的感染，这表明组织蛋白酶B是Eco - MLV感染所必需的。然而，NIH3T3细胞中的组织蛋白酶B活性呈阴性或极低。CA - 074Me是如何减弱Eco - MLV感染的呢？用CA - 074Me处理NIH3T3细胞可抑制组织蛋白酶L的活性，并且一种组织蛋白酶L特异性抑制剂CLIK148可减弱Eco - MLV载体的感染。这些结果表明，CA - 074Me对组织蛋白酶L活性的抑制导致了Eco - MLV感染的抑制，这表明组织蛋白酶L是NIH3T3细胞中Eco - MLV感染所必需的。用CA - 074Me处理可抑制表达外源性小鼠嗜亲性受体以及内源性组织蛋白酶B和L的人细胞中的Eco - MLV感染，但用CLIK148处理则不能，这表明仅CLIK148对组织蛋白酶L的抑制不足以防止在同时表达组织蛋白酶B和L的细胞中发生Eco - MLV感染，而CA - 074Me通过抑制组织蛋白酶B和L两者来抑制Eco - MLV感染。这些结果表明，组织蛋白酶B或L中的任何一种对于Eco - MLV感染都是足够的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/b62845f43a85/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/7afe1de87655/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/f879e89bb185/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/803470141de0/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/57d5410a47ba/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/b62845f43a85/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/7afe1de87655/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/f879e89bb185/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/803470141de0/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/57d5410a47ba/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1138/7111982/b62845f43a85/gr5_lrg.jpg

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