Hawkins N C, McGhee J D
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Nucleic Acids Res. 1990 Oct 25;18(20):6101-6. doi: 10.1093/nar/18.20.6101.
We designed a unique 36-mer oligonucleotide probe, based on the most highly conserved amino acid sequences of Antennapedia-like homeodomains and the codon bias of Caenorhabditis elegans. This probe was then used to isolate four classes of genes from a C. elegans genomic library. Sequencing reveals that we have isolated three new homeobox genes, designated ceh-1, ceh-9 and ceh-10. The fourth homeobox gene, ceh-11, has recently been described by Schaller et al (Nucleic Acids Res. 18, 2033-2036). The amino acid sequence of ceh-1 is 87% similar to the honeybee H40 homeodomain, 85% similar to the Drosophila NK-1 homeodomain and 82% similar to the chicken CHox3 homeodomain. The sequence ceh-10 appears to be a member of the paired class of homeodomains. The other two sequences, ceh-9 and ceh-11, remain unclassified. Three of the four sequences have at least one intron within the homeobox region. Transcripts of ceh-10 and ceh-11 are present in embryonic RNA but are greatly diminished in later developmental stages. Three of the four new genes have been placed on the C. elegans genomic map.
我们基于触角足样同源结构域的高度保守氨基酸序列和秀丽隐杆线虫的密码子偏好性，设计了一种独特的36聚体寡核苷酸探针。然后用该探针从秀丽隐杆线虫基因组文库中分离出四类基因。测序结果显示，我们分离出了三个新的同源异型框基因，命名为ceh-1、ceh-9和ceh-10。第四个同源异型框基因ceh-11，最近已由沙勒等人描述（《核酸研究》18卷，2033 - 2036页）。ceh-1的氨基酸序列与蜜蜂H40同源结构域相似度为87%，与果蝇NK-1同源结构域相似度为85%，与鸡CHox3同源结构域相似度为82%。ceh-10序列似乎是同源结构域配对类别的一个成员。另外两个序列ceh-9和ceh-11仍未分类。四个序列中的三个在同源异型框区域内至少有一个内含子。ceh-10和ceh-11的转录本存在于胚胎RNA中，但在后期发育阶段大幅减少。四个新基因中的三个已定位到秀丽隐杆线虫基因组图谱上。
