Wang Ping, Chen Shanshan, Zhu Xingyi, Xie Jie
College of Pharmaceutical Sciences, Zhejiang University of Technology, Key Laboratory of Pharmaceutical Engineering of Ministry of Education, Hangzhou 310014, China.
J Chromatogr A. 2009 Nov 6;1216(45):7639-44. doi: 10.1016/j.chroma.2009.08.093. Epub 2009 Sep 9.
Daidzein-imprinted poly(methacrylamide-co-acrylic acid) composite membranes with different ratios of methacrylamide (MAM) versus acrylic acid (AA) were prepared via UV initiated photo-copolymerization on the commercial filter paper with ethylene glycol dimethacrylate (EGDMA) as cross-linker and mixed cellulose ester as agglutinant. Infra-red (IR) spectroscopy and scanning electron microscope (SEM) were used to visualize the surface of the membranes. Binding and recognising properties of the imprinted composite membranes to daidzein and its analogues genistein were evaluated by static adsorption experiment. It was found that the daidzein-imprinted membranes showed high selectivity to daidzein, with the highest selectivity when the composite membrane with the ratio of MAM vs AA as 4:1. The results suggested that the molecularly imprinted composite membranes were potentially useful for daidzein enrichment.
