Zhu Xing-Yi, Zheng Zhang-Juan, Xie Jie, Wang Ping
College of Pharmaceutical Sciences, Zhejiang University of Technology, Key Laboratory of Pharmaceutical Engineering of Ministry of Education, Hangzhou, P. R. China.
J Sep Sci. 2012 Jan;35(2):315-9. doi: 10.1002/jssc.201100731. Epub 2011 Dec 9.
Molecularly imprinted membranes (MIMs) for selective separation of magnolol were prepared by thermal polymerization using magnolol as the template, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, 2,2-azobisisobutyronitrile (AIBN) as the initiator, organic solvent as the porogen, methacrylamide (MAM) and acrylic acid (AA) as the functional monomers and cellulose acetate as the agglutinant. Commercial filter paper was used as the supporting material. The effects of different porogens and the ratio of functional monomers on the binding and recognition capacity of MIMs were investigated, and the morphology of the membranes was examined by scanning electron microscopy (SEM). The results showed that the MIMs have the highest selectivity to magnolol when the ratio of MAM/AA was 1:4 and tetrahydrofuran (THF) with dimethyl sulfoxide (DMSO) was used as the porogen. The morphology of the imprinted membranes after template extracting is much rougher with big cavities than that of the non-imprinted membranes (NIMs) and the imprinted membranes before template extracting. The MIMs can selectively separate the magnolol.
以厚朴酚为模板，乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，2,2-偶氮二异丁腈（AIBN）为引发剂，有机溶剂为致孔剂，甲基丙烯酰胺（MAM）和丙烯酸（AA）为功能单体，醋酸纤维素为粘结剂，通过热聚合制备了用于选择性分离厚朴酚的分子印迹膜（MIMs）。使用商用滤纸作为支撑材料。研究了不同致孔剂和功能单体比例对MIMs结合和识别能力的影响，并通过扫描电子显微镜（SEM）观察了膜的形态。结果表明，当MAM/AA比例为1:4且使用四氢呋喃（THF）与二甲基亚砜（DMSO）作为致孔剂时，MIMs对厚朴酚具有最高的选择性。模板去除后的印迹膜形态比非印迹膜（NIMs）和模板去除前的印迹膜更加粗糙，有大的孔洞。MIMs能够选择性地分离厚朴酚。
