Qin Peiyong, Yang Jie, Karim M Nazmul
Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing, People's Republic of China.
Scanning. 2011 Jan-Feb;33(1):7-12. doi: 10.1002/sca.20212. Epub 2011 Jan 18.
Molecular-imprinted membranes of Puerarin were prepared by phase inversion technique with acrylonitrile-acrylic acid copolymer (P (AN-co-AA)). To characterize P (AN-co-AA), ubbelohde viscometer was used to measure its viscosity-molecular weight. P (AN-co-AA) with different molecular weight was used to prepare membranes. The copolymer-dimethyl sulfoxide solution with Puerarin (PU) template was coagulated in water at various temperatures. The increase in P (AN-co-AA) molecular weight and the decrease in coagulation temperature caused an increase in PU recognition property of the resultant membrane. The PU imprinted membrane prepared with P (AN-co-AA) showed good selective ability to PU. The purity of PU increased from 56.51 to 98.41 wt%. Surface and cross-section morphology of the membranes were analyzed by using scanning electron micrograph. High-performance liquid chromatography was used for the quantification of Puerarin in isolated fraction.
采用相转化技术，以丙烯腈-丙烯酸共聚物（P(AN-co-AA)）制备了葛根素分子印迹膜。为表征P(AN-co-AA)，使用乌氏粘度计测量其粘均分子量。采用不同分子量的P(AN-co-AA)制备膜。将含有葛根素（PU）模板的共聚物-二甲基亚砜溶液在不同温度的水中凝固。P(AN-co-AA)分子量的增加和凝固温度的降低导致所得膜对PU的识别性能增强。用P(AN-co-AA)制备的PU印迹膜对PU表现出良好的选择性。PU的纯度从56.51 wt%提高到98.41 wt%。利用扫描电子显微镜分析了膜的表面和横截面形态。采用高效液相色谱法对分离组分中的葛根素进行定量分析。
