Ahmed W, Goonetilleke A, Powell D, Gardner T
Department of Natural Resources and Water, 80 Meiers Road, Indooroopilly, Sunshine Coast, QLD 4558, Brisbane 4068, Australia.
Water Res. 2009 Nov;43(19):4872-7. doi: 10.1016/j.watres.2009.08.042. Epub 2009 Sep 3.
The host specificity of the five published sewage-associated Bacteroides markers (i.e., HF183, BacHum, HuBac, BacH and Human-Bac) was evaluated in Southeast Queensland, Australia by testing fecal DNA samples (n=186) from 11 animal species including human fecal samples collected via influent to a sewage treatment plant (STP). All human fecal samples (n=50) were positive for all five markers indicating 100% sensitivity of these markers. The overall specificity of the HF183 markers to differentiate between humans and animals was 99%. The specificities of the BacHum and BacH markers were>94%, suggesting that these markers are suitable for the detection of sewage pollution in environmental waters in Australia. The HuBac (i.e., 63%) and Human-Bac (i.e., 79% specificity) markers performed poorly in distinguishing between the sources of human and animal fecal samples. It is recommended that the specificity of the sewage-associated markers must be rigorously tested prior to its application to identify the sources of fecal pollution in environmental waters.
通过检测来自11种动物的粪便DNA样本（n = 186），包括通过污水处理厂（STP）进水口收集的人类粪便样本，在澳大利亚昆士兰州东南部评估了已发表的五种与污水相关的拟杆菌标记物（即HF183、BacHum、HuBac、BacH和Human - Bac）的宿主特异性。所有人类粪便样本（n = 50）对所有五种标记物均呈阳性，表明这些标记物的敏感性为100%。HF183标记物区分人类和动物的总体特异性为99%。BacHum和BacH标记物的特异性>94%，表明这些标记物适用于检测澳大利亚环境水体中的污水污染。HuBac（即63%）和Human - Bac（即特异性为79%）标记物在区分人类和动物粪便样本来源方面表现不佳。建议在将与污水相关的标记物应用于识别环境水体中粪便污染来源之前，必须对其特异性进行严格测试。
