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聚合酶链反应（PCR）数据及微生物源追踪遗传标记物的比较性能

PCR data and comparative performance of microbial source tracking genetic markers.

作者信息

Somnark Pornjira, Chyerochana Natcha, Kongprajug Akechai, Mongkolsuk Skorn, Sirikanchana Kwanrawee

机构信息

Applied Biological Sciences, Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Lak Si, Bangkok 10210 Thailand.

Research Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, Bangkok 10210 Thailand.

出版信息

Data Brief. 2018 May 5;19:156-169. doi: 10.1016/j.dib.2018.04.129. eCollection 2018 Aug.

DOI:10.1016/j.dib.2018.04.129
PMID:29892629
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5992956/
Abstract

We reported modified endpoint PCR results analyzed by universal and human-, swine-, and cattle-specific gene markers with human sewage and animal fecal samples (i.e., swine, cattle, chicken, goat, sheep, buffalo, and duck) from Tha Chin and Chao Phraya watersheds. Annealing locations of PCR primers were illustrated by maps of 16s rRNA genes. We also summarized previously published work on the performance of the PCR assays. For further discussion of the data presented here, please refer to Somnark et al., Performance evaluation of genetic markers for human and animal microbial source tracking in tropical agricultural watersheds, Environ. Pollut. 236 (2018) 100-110.

摘要

我们报告了通过通用以及人类、猪和牛特异性基因标记对泰国湄南河流域和湄南河流域的人类污水和动物粪便样本（即猪、牛、鸡、山羊、绵羊、水牛和鸭）进行分析的改良终点PCR结果。PCR引物的退火位置通过16s rRNA基因图谱进行了说明。我们还总结了先前发表的关于PCR检测性能的工作。有关此处呈现的数据的进一步讨论，请参考Somnark等人的《热带农业流域人类和动物微生物源追踪遗传标记的性能评估》，《环境污染》236（2018）100 - 110。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70d7/5992956/be83608ab97c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70d7/5992956/6dfb4c430d8f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70d7/5992956/028295c8a446/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70d7/5992956/be83608ab97c/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70d7/5992956/6dfb4c430d8f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70d7/5992956/028295c8a446/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70d7/5992956/be83608ab97c/gr3.jpg

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Tracking the primary sources of fecal pollution in a tropical watershed in a one-year study.在一项为期一年的研究中追踪热带流域粪便污染的主要来源。
Appl Environ Microbiol. 2013 Mar;79(5):1689-96. doi: 10.1128/AEM.03070-12. Epub 2013 Jan 4.
3
Consistency in the host specificity and host sensitivity of the Bacteroides HF183 marker for sewage pollution tracking.
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Lett Appl Microbiol. 2012 Oct;55(4):283-9. doi: 10.1111/j.1472-765X.2012.03291.x. Epub 2012 Aug 17.
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Effect of liquid whey feeding on fecal microbiota of mature and growing pigs.液体乳清喂养对成熟和生长猪粪便微生物群的影响。
Anim Sci J. 2011 Aug;82(4):607-15. doi: 10.1111/j.1740-0929.2011.00876.x. Epub 2011 Apr 20.
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