Department of Environment and Resource Management, Indooroopilly, Brisbane, Australia.
Can J Microbiol. 2010 Oct;56(10):838-45. doi: 10.1139/w10-070.
This paper aimed to assess the magnitude of sewage pollution in an urban lake in Dhaka, Bangladesh, by using quantitative PCR of sewage-associated Bacteroides HF183 markers. PCR was also used for the quantitative detection of ruminant wastewater-associated CF128 markers along with the enumeration of traditional fecal indicator bacteria, namely enterococci. The number of enterococci in lake water samples ranged from 1.1 × 10⁴ to 1.9 × 10⁵ colony-forming units/100 mL water. From the 20 water samples tested, 14 (70%) and 7 (35%) were PCR positive for HF183 and CF128 markers, respectively. The numbers of HF183 and CF128 markers in lake water samples were 3.9 × 10⁴ to 6.3 × 10⁷ and 9.3 × 10³ to 6.3 × 10⁵ genomic units/100 mL water, respectively. The high numbers of enterococci and HF183 markers are indicative of sewage pollution and potential health risks to those who use the lake water for nonpotable purposes such as bathing and washing clothes. This is the first study that investigated the presence of microbial source tracking markers in Dhaka, Bangladesh, where diarrhoeal disease is one of the major causes of childhood mortality. The molecular assay used in this study can provide valuable information on the extent of sewage pollution, thus facilitating the development of robust strategies to minimize potential health risks.
本研究旨在通过定量聚合酶链式反应（PCR）检测污水相关的拟杆菌 HF183 标志物，评估孟加拉国达卡市一个城市湖泊的污水污染程度。PCR 还用于定量检测反刍动物污水相关的 CF128 标志物，以及传统粪便指示菌——肠球菌的计数。湖水中肠球菌的数量范围为 1.1×10⁴至 1.9×10⁵ 个菌落形成单位/100 毫升水。在测试的 20 个水样中，HF183 和 CF128 标志物的 PCR 阳性率分别为 14（70%）和 7（35%）。湖水中 HF183 和 CF128 标志物的数量分别为 3.9×10⁴至 6.3×10⁷ 和 9.3×10³至 6.3×10⁵ 基因组单位/100 毫升水。肠球菌和 HF183 标志物数量高表明存在污水污染，且湖泊水被用于非饮用水目的（如洗澡和洗衣服）时，可能会对使用者的健康造成潜在风险。这是首次在孟加拉国达卡市调查微生物溯源标志物存在情况的研究，腹泻病是导致儿童死亡的主要原因之一。本研究中使用的分子检测方法可以提供有关污水污染程度的有价值信息，从而有助于制定减少潜在健康风险的稳健策略。
