Laboratory of Theriogenology, Iwate University, Morioka, 020-8550, Japan.
Theriogenology. 2009 Dec;72(9):1153-62. doi: 10.1016/j.theriogenology.2009.07.007. Epub 2009 Sep 23.
The uterine condition of clinically normal postpartum Holstein-Friesian dairy cows (n = 45) was evaluated once weekly (Weeks 3 to 7) by endometrial cytology, vaginal mucus collection device (VMCD), vaginoscopy, and ultrasonography to establish a relationship with postpartum resumption of ovulatory cycles. The time of first detection of the corpus luteum (CL) by ultrasonography and plasma progesterone concentration >or=1 ng/mL was recorded. By 49 d postpartum, 78% of the cows (n=35) had resumed ovarian function (CL group), whereas the remainder (n=10) had no CL (NCL group). There was a positive correlation between VMCD score and presence of fluid in the uterus in cows with a CL (P<0.01) during Week 3 postpartum but no significant correlation in cows without a CL. Percentage of polymorphonuclear leukocytes (PMN%) was higher in the NCL group (mean+/-SEM, 24.6+/-9.4%) than in the CL group (11.7+/-2.2%) during Week 5 postpartum (P<0.05). The PMN% (4.5+/-6.5%) and VMCD (0.5+/-0.5) scores during Week 5 in cows ovulating by Day 28 were lower (P<0.01) than the PMN% (15.0+/-14.3%) and VMCD (1.1+/-0.9) scores in those ovulating by Day 49. In conclusion, higher PMN% at 5 wk postpartum was associated with delayed resumption of ovarian cyclicity in high-producing dairy cows.
临床正常的产后荷斯坦奶牛（n = 45）的子宫状况每周（第 3 至 7 周）通过子宫内膜细胞学、阴道黏液收集器（VMCD）、阴道镜和超声检查进行评估，以建立与产后排卵周期恢复的关系。记录超声检测到黄体（CL）的时间和血浆孕酮浓度>或=1ng/mL。产后 49 天，78%的奶牛（n=35）恢复了卵巢功能（CL 组），而其余的奶牛（n=10）没有 CL（NCL 组）。在产后第 3 周，CL 奶牛的 VMCD 评分与子宫内液体存在呈正相关（P<0.01），但在无 CL 的奶牛中无显著相关性。在产后第 5 周，NCL 组的多形核白细胞（PMN%）百分比（平均+/-SEM，24.6+/-9.4%）高于 CL 组（11.7+/-2.2%）（P<0.05）。在第 5 周排卵至第 28 天的奶牛中，PMN%（4.5+/-6.5%）和 VMCD（0.5+/-0.5）评分低于排卵至第 49 天的奶牛（PMN%（15.0+/-14.3%）和 VMCD（1.1+/-0.9）评分（P<0.01）。总之，产后第 5 周较高的 PMN%与高产奶牛卵巢周期性恢复延迟有关。
