Ghanem Mohamed Elshabrawy, Tezuka Erisa, Devkota Bhuminand, Izaike Yoshiaki, Osawa Takeshi
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University 020-8550, Japan; Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
J Reprod Dev. 2015;61(1):54-60. doi: 10.1262/jrd.2014-051. Epub 2014 Dec 6.
We investigated the relationship between the persistence of uterine bacterial infections with cytologically determined endometritis and ovarian function in 65 postpartum Holstein cows. Vaginal mucus discharges were collected, and endometrial smear samples (n = 130) were collected for cytological and bacteriological examinations from the cows at weeks 5 and 7 postpartum (pp). Blood samples were collected at weeks 3, 5 and 7 pp to determine plasma progesterone concentrations to monitor ovarian activity. According to the bacteriological examination, cows were classified into four groups. The first group (n = 32; 49%) comprised cows negative for bacteria at weeks 5 and 7 pp. The second group (n = 11; 17%) comprised cows with bacterial infections at week 5 pp but that were clear of infection at week 7 pp. The third group (n = 12; 19%) comprised cows without bacteria at week 5 pp but that acquired an infection by week 7 pp. The fourth group (n = 10; 15%) comprised cows with bacterial infections at weeks 5 and 7 pp (persistence of infection). A positive correlation (P < 0.001) was noted between the severity of cytologically determined endometritis, purulent vaginal discharge and the persistence of infection. Cows with persistent infections had a significantly (P < 0.01) prolonged luteal phase compared with cows without infection. In conclusion, the prevalence of cytologically determined endometritis and prolonged luteal phase were significantly increased in cows with persistent infections.
我们研究了65头产后荷斯坦奶牛子宫细菌感染的持续性与细胞学诊断的子宫内膜炎及卵巢功能之间的关系。在产后第5周和第7周收集阴道黏液分泌物，并采集子宫内膜涂片样本（n = 130）进行细胞学和细菌学检查。在产后第3周、第5周和第7周采集血样，测定血浆孕酮浓度以监测卵巢活动。根据细菌学检查，将奶牛分为四组。第一组（n = 32；49%）包括产后第5周和第7周细菌检测呈阴性的奶牛。第二组（n = 11；17%）包括产后第5周有细菌感染但在第7周感染清除的奶牛。第三组（n = 12；19%）包括产后第5周无细菌感染但在第7周感染的奶牛。第四组（n = 10；15%）包括产后第5周和第7周均有细菌感染（感染持续）的奶牛。细胞学诊断的子宫内膜炎严重程度、脓性阴道分泌物与感染持续性之间存在正相关（P < 0.001）。与未感染的奶牛相比，感染持续的奶牛黄体期显著延长（P < 0.01）。总之，感染持续的奶牛细胞学诊断的子宫内膜炎患病率和黄体期延长显著增加。
