Senosy W, Uchiza M, Tameoka N, Izaike Y, Osawa T
Laboratory of Theriogenology, Iwate University, Morioka, Japan.
Reprod Domest Anim. 2011 Oct;46(5):800-6. doi: 10.1111/j.1439-0531.2010.01744.x. Epub 2011 Jan 17.
The effect of ovarian predominating structures and uterine condition on the result of some diagnostic tools for the evaluation of endometritis was studied in postpartum (pp) Holstein-Friesian dairy cows (n = 58). Endometrial cytology (EC) and the evaluation of vaginal mucus by vaginoscopy or Metricheck were performed weekly from week 3 to 7 pp. The ovarian studies involved the predominating structures including cystic follicles with plasma progesterone (P(4) ; more or <1 ng/ml; >23 mm), corpus luteum (CL), pre-ovulatory follicles (10-23 mm) and small follicles (<10 mm). The uterine conditions comprised uterine involution, tonicity and fluid in uterus (FIU) regarding echogenicity extent. During week 5, the percentage of polymorphonuclear neutrophils (PMN%) was higher (p < 0.05) in animals with pre-ovulatory follicles (mean ± SEM, 26.3 ± 7.6%) than animals having CL (11.0 ± 3.6%). In cystic ovaries, during week 5, PMN% was higher (p < 0.05) in follicular cysts with low progesterone (P(4) < 1 ng/ml; 9.3 ± 2.6%) than those with high P(4) (P(4) ≥ 1 ng/ml; 1.5 ± 1.1%). Moreover, PMN% was higher (p < 0.01) in animals with non-involuted uterus (11.5 ± 7.4%) than those with involuted uterus (2.7 ± 0.6%) during week 7 pp. The animals that had abnormal mucus determined by Metricheck was higher in animals with atonic uterus than those with tonic uterus during week 6 (82.6% vs 51.5%; p < 0.05) and 7 (71.4% vs 25.7%; p < 0.01) pp. In addition, by vaginoscopy, the prevalence of animals with abnormal discharge showing small follicles (100%, 5/5) during week 3 pp and pre-ovulatory follicles (40.0%, 8/20) during week 5 pp was higher (p < 0.05) when compared to those having CL during week 3 (33.3%; 1/3) and week 5 pp (7.7%; 2/26), respectively. In conclusion, endometrial cytology, Metricheck and vaginoscopy were influenced by the predominating various ovarian structures and uterine condition in early pp high-yielding dairy cows.
在产后荷斯坦 - 弗里生奶牛（n = 58）中，研究了卵巢主导结构和子宫状况对一些用于评估子宫内膜炎的诊断工具结果的影响。从产后第3周开始至第7周，每周进行子宫内膜细胞学检查（EC）以及通过阴道镜检查或Metricheck评估阴道黏液。卵巢研究涉及主导结构，包括伴有血浆孕酮（P(4)；大于或小于1 ng/ml；大于23 mm）的卵泡囊肿、黄体（CL）、排卵前卵泡（10 - 23 mm）和小卵泡（小于10 mm）。子宫状况包括子宫复旧、张力以及子宫内液体（FIU）的回声程度。在第5周时，有排卵前卵泡的动物（平均值±标准误，26.3±7.6%）中多形核中性粒细胞百分比（PMN%）高于有黄体的动物（11.0±3.6%）（p < 0.05）。在第5周时，孕酮水平低（P(4) < 1 ng/ml；9.3±2.6%）的卵泡囊肿中的PMN%高于孕酮水平高（P(4)≥1 ng/ml；1.5±1.1%）的卵泡囊肿（p < 0.05）。此外，在产后第7周时，子宫未复旧动物（11.5±7.4%）中的PMN%高于子宫已复旧动物（2.7±0.6%）（p < 0.01）。在第6周（82.6%对51.5%；p < 0.05）和第7周（71.4%对25.7%；p < 0.01）产后，Metricheck检测出黏液异常的动物中，子宫张力低的动物比子宫张力正常的动物更多。另外通过阴道镜检查，产后第3周小卵泡动物（100%；5/5）和第5周排卵前卵泡动物（40.0%；8/20）出现异常分泌物的比例高于第3周有黄体的动物（33.3%；1/3）和第5周有黄体的动物（7.7%；2/26）（p < 0.05）。总之，在产后早期高产奶牛中，子宫内膜细胞学检查、Metricheck和阴道镜检查受各种卵巢主导结构和子宫状况的影响。
