利用磁阱进行的单DNA/蛋白质研究。

Single DNA/protein studies with magnetic traps.

作者信息

Meglio Adrien, Praly Elise, Ding Fangyuan, Allemand Jean-Francois, Bensimon David, Croquette Vincent

机构信息

ENS, LPS, UMR 8550 CNRS, 24 rue Lhomond, 75005 Paris, France; Universite Paris 6, Universite Paris 7, 4 place Jussieu, 75005 Paris, France.

出版信息

Curr Opin Struct Biol. 2009 Oct;19(5):615-22. doi: 10.1016/j.sbi.2009.08.005. Epub 2009 Sep 23.

DOI:10.1016/j.sbi.2009.08.005

PMID:19783425

Abstract

Magnetic traps provide a simple technique to pull and twist a variety of biomolecules and monitor the resulting change in extension. They have been used with great success to investigate the interaction of stretched and supercoiled DNA and DNA fibers (e.g. chromatin) with a great variety of enzymes. In this small review we will address their recent use in the study of topoisomerases, gyrase, DNA translocases and various structural proteins.

摘要

磁阱提供了一种简单的技术，用于牵拉和扭转各种生物分子，并监测由此产生的伸展变化。它们已被成功用于研究拉伸和超螺旋DNA以及DNA纤维（如染色质）与多种酶的相互作用。在这篇简短的综述中，我们将探讨它们最近在拓扑异构酶、促旋酶、DNA转位酶和各种结构蛋白研究中的应用。