Chen S, Gluhak-Heinrich J, Wang Y H, Wu Y M, Chuang H H, Chen L, Yuan G H, Dong J, Gay I, MacDougall M
Department of Pediatric Dentistry, TheUniversity of Texas Health Science Center at San Antonio,7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA.
J Dent Res. 2009 Oct;88(10):904-9. doi: 10.1177/0022034509342873.
The transcription factors Runx2 and Osx are necessary for osteoblast and odontoblast differentiation, while Dspp is important for odontoblast differentiation. The relationship among Runx2, Osx, and Dspp during tooth and craniofacial bone development remains unknown. In this study, we hypothesized that the roles of Runx2 and Osx in the regulation of osteoblast and odontoblast lineages may be independent of one another. The results showed that Runx2 expression overlapped with Osx in dental and osteogenic mesenchyme from E12 to E16. At the later stages, from E18 to PN14, Runx2 and Osx expressions remained intense in alveolar bone osteoblasts. However, Runx2 expression was down-regulated, whereas Osx expression was clearly seen in odontoblasts. At later stages, Dspp transcription was weakly present in osteoblasts, but strong in odontoblasts where Osx was highly expressed. In mouse odontoblast-like cells, Osx overexpression increased Dspp transcription. Analysis of these data suggests differential biological functions of Runx2, Osx, and Dspp during odontogenesis and osteogenesis.
转录因子Runx2和Osx对成骨细胞和成牙本质细胞的分化是必需的，而Dspp对成牙本质细胞的分化很重要。Runx2、Osx和Dspp在牙齿和颅面骨发育过程中的关系尚不清楚。在本研究中，我们假设Runx2和Osx在调节成骨细胞和成牙本质细胞谱系中的作用可能彼此独立。结果显示，从胚胎第12天（E12）到第16天（E16），Runx2的表达与Osx在牙源性和成骨间充质中重叠。在后期，从胚胎第18天（E18）到出生后第14天（PN14），Runx2和Osx在牙槽骨成骨细胞中表达仍然强烈。然而，Runx2的表达下调，而在成牙本质细胞中可清楚地看到Osx的表达。在后期，Dspp转录在成骨细胞中较弱，但在Osx高表达的成牙本质细胞中较强。在小鼠成牙本质细胞样细胞中，Osx过表达增加了Dspp转录。对这些数据的分析表明Runx2、Osx和Dspp在牙发生和成骨过程中具有不同的生物学功能。
