Department of Otorhinolaryngology, Kyung Hee University School of Medicine, Seoul, Korea.
Clin Exp Otorhinolaryngol. 2009 Sep;2(3):131-5. doi: 10.3342/ceo.2009.2.3.131. Epub 2009 Sep 23.
Inadequate antibody responses to pathogens may lead to the recurrence of otitis media with effusion (OME). Although B-cell production by antibodies is controlled by transcription factors, the status of these factors has not been assessed in patients with OME.
Expression of immunoglobulin was measured by enzyme-linked immunosorbent assay. Expression of transcription factors Bcl-6, Blimp-1, Pax-5, and XBP-1 was assessed by RT-PCR in the middle-ear fluid of 29 children with >4 OME episodes in 12 months or >3 episodes in 6 months (the OME-prone group) and in 32 children with <3 OME episodes in 12 months (OME group). The relationship between recurrence of OME and expression levels of immunoglobulins and transcription factors in middle-ear fluid was determined.
The concentration of IgA in middle-ear fluid was significantly lower in the OME-prone than in the OME group, as was the expression of mRNA encoding the transcription factors Blimp-1 and XBP-1 (P<0.05 each). Expression of mRNA encoding the transcription factors Bcl-6 and Pax-5 was more intense in the OME-prone than in the OME group, but these differences were not significant (P>0.05).
Lower concentrations of IgA, Blimp-1 and XBP-1 in middle ear fluid of patients with OME may be related to OME recurrence and chronicity.
针对病原体的抗体反应不足可能导致分泌性中耳炎（OME）复发。尽管抗体的 B 细胞产生受转录因子控制，但这些因子在 OME 患者中的状态尚未得到评估。
通过酶联免疫吸附试验测量免疫球蛋白的表达。通过 RT-PCR 评估 29 例在 12 个月内发生>4 次 OME 发作或在 6 个月内发生>3 次发作的 OME 易患组（OME 易患组）和 32 例在 12 个月内发生<3 次 OME 发作的儿童（OME 组）中耳液中的转录因子 Bcl-6、Blimp-1、Pax-5 和 XBP-1 的表达。确定 OME 复发与中耳液中免疫球蛋白和转录因子表达水平之间的关系。
OME 易患组中耳液中 IgA 的浓度明显低于 OME 组，转录因子 Blimp-1 和 XBP-1 的 mRNA 表达也降低（P<0.05）。OME 易患组转录因子 Bcl-6 和 Pax-5 的 mRNA 表达强度高于 OME 组，但差异无统计学意义（P>0.05）。
OME 患者中耳液中 IgA、Blimp-1 和 XBP-1 浓度较低可能与 OME 复发和慢性化有关。
