Skotnicka B, Hassmann E
Department of Pediatric Otorhinolaryngology, University Children's Hospital, Bialystok, Poland.
Eur Arch Otorhinolaryngol. 2000;257(6):323-6. doi: 10.1007/s004059900218.
We assayed 38 middle ear effusions from 23 children aged 4-13 years (mean 7) undergoing tympanostomy tube placements. All fluid was assayed for tumor necrosis factor (TNF) alpha, interleukin (IL) 1beta, IL-8, and IL-10. Cytokine concentrations were measured by means of an enzyme-linked immunosorbent assay. Detectable levels of IL-1beta, IL-8, and IL-10 were found in all of the effusions. TNF-alpha was detected in 18 of the middle ear effusions (47.4%). The mean concentration of TNF-alpha, IL-1beta , IL-8, and IL-10 was, respectively, 0.423 +/- 1.39, 30.58 +/- 68.7, 7001.9 +/- 6743, and 56 +/- 58.7 pg/ml. There was a strong, statistically significant correlation between the concentrations of TNF-alpha and IL-1beta (r = 0.87, P = 0.001) and between IL-1beta and IL-8 (r = 0.53, P = 0.001). There was no correlation between the concentrations of IL-10 and other cytokines examined or between tympanic membrane pathology and the concentrations of TNF-alpha, IL-1beta , IL-8, or IL-10. The presence of IL-10 in middle ear effusions may be one of the causes of a lack of clinical features of acute inflammation and may lead to a chronic inflammatory state.
我们对23名年龄在4至13岁（平均7岁）接受鼓膜置管术的儿童的38份中耳积液进行了检测。对所有积液检测肿瘤坏死因子（TNF）α、白细胞介素（IL）1β、IL - 8和IL - 10。通过酶联免疫吸附测定法测量细胞因子浓度。在所有积液中均发现了可检测水平的IL - 1β、IL - 8和IL - 10。在18份中耳积液（47.4%）中检测到了TNF -α。TNF -α、IL - 1β、IL - 8和IL - 10的平均浓度分别为0.423±1.39、30.58±68.7、7001.9±6743和56±58.7 pg/ml。TNF -α与IL - 1β的浓度之间（r = 0.87，P = 0.001）以及IL - 1β与IL - 8的浓度之间（r = 0.53，P = 0.001）存在强的、具有统计学意义的相关性。IL - 10与所检测的其他细胞因子的浓度之间，或鼓膜病理学与TNF -α、IL - 1β、IL - 8或IL - 10的浓度之间均无相关性。中耳积液中IL - 10的存在可能是缺乏急性炎症临床特征的原因之一，并可能导致慢性炎症状态。
