Settin Ahmad, Hassan Hanaa, El-Baz Rizk, Hassan Tahia
Genetics Unit, Children Hospital, Mansoura University, Egypt, Visiting Prof. Qassim University, BO 6655 Buraydah 51452, Saudi Arabia.
Acta Dermatovenerol Alp Pannonica Adriat. 2009 Sep;18(3):105-12.
Psoriasis is a chronic inflammatory skin disease with an immunogenetic background. This study was planned to check for the association of polymorphisms related to cytokine genes TNF-alpha-308(G/A), IL-10-1082(G/A), IL-6-174(G/C), and IL-1Ra (VNTR) with psoriasis in cases from Egypt.
This study included 46 cases with psoriasis recruited from the Department of Dermatology, University Hospitals, Nile Delta region of Egypt. They included 14 males and 32 females with a mean age +/- SD of 46.68 +/- 12.16 years and a range of 15 to 70 years. Their genotypes were compared to 98 healthy controls of matched age and sex from the same locality. Genotyping was done through DNA amplification using PCR with sequence-specific primers for polymorphic alleles.
Compared to controls, cases showed a significantly higher frequency of certain genotypes including IL-6-174 CC (p < 0.001, OR = 6.7), IL-10-1082 GG (p < 0.05, OR = 5.1), and TNF-alpha-308 GG (p < 0.05, OR = 3.7). Combined heterozygosity for IL-10 GA, IL-6 GC, and TNF GA showed a significant low frequency among the cases studied.
Genetic polymorphisms related to the IL6, IL10, and TNF-alpha genes showed a particular pattern of association with psoriasis that may have a potential impact on disease counseling and management.
银屑病是一种具有免疫遗传背景的慢性炎症性皮肤病。本研究旨在检测埃及患者中细胞因子基因TNF-α -308（G/A）、IL-10 -1082（G/A）、IL-6 -174（G/C）和IL-1Ra（VNTR）的多态性与银屑病的相关性。
本研究纳入了46例来自埃及尼罗河三角洲地区大学医院皮肤科的银屑病患者。其中包括14名男性和32名女性，平均年龄±标准差为46.68±12.16岁，年龄范围为15至70岁。将他们的基因型与来自同一地区年龄和性别匹配的98名健康对照进行比较。通过使用针对多态性等位基因的序列特异性引物进行PCR扩增来进行基因分型。
与对照组相比，病例组中某些基因型的频率显著更高，包括IL-6 -174 CC（p < 0.001，OR = 6.7）、IL-10 -1082 GG（p < 0.05，OR = 5.1）和TNF-α -308 GG（p < 0.05，OR = 3.7）。IL-10 GA、IL-6 GC和TNF GA的联合杂合性在研究的病例组中显示出显著低频率。
与IL6、IL10和TNF-α基因相关的基因多态性显示出与银屑病的特定关联模式，这可能对疾病咨询和管理具有潜在影响。
