Settin Ahmad A, Hassan Hanaa A, El-Baz Rizk A, Hassan Tahia A
College of Medicine, Qassim University, Qassim, Saudi Arabia.
Indian J Dermatol. 2011 May;56(3):272-7. doi: 10.4103/0019-5154.82479.
Psoriasis is a chronic inflammatory skin disease with an immunogenetic background. This work was planned to check for the association of polymorphisms related to cytokine genes TNF-α(-308) (G/A), IL-10(-1082) (G/A), IL-6(-174) (G/C), and IL-1Ra (VNTR) with psoriasis in cases from Egypt.
This work included 46 cases with psoriasis recruited from the Dermatology Departments, University Hospitals, Nile Delta region of Egypt. They included 14 males and 32 females with an age mean ± SD of 46.68 ± 12.16 years and range of 15-70 years. Their genotypes were compared to 98 healthy controls of matched age and sex from the same locality. Genotyping was done through deoxyribonucleic acid amplification using PCR with sequence specific primers for polymorphic alleles.
Compared to controls, cases showed significant higher frequency of certain genotypes including IL-6(-174) CC (P < 0.001, OR = 6.7), IL-10(-1082) GG (P < 0.05, OR = 5.1), and TNF-α(-308) GG (P < 0.05, OR = 3.7). TNF-α(-308) GG and IL-10(-1082) GG genotypes were higher among cases with plaque subtype of moderate severity. Combined heterozygosity for IL-10 GA, IL-6 GC with TNF GA showed a significant low frequency among studied cases.
Genetic polymorphisms related to IL6, IL10, and TNF-α genes showed a particular pattern of association with psoriasis that may have a potential impact on disease counseling and management.
银屑病是一种具有免疫遗传背景的慢性炎症性皮肤病。本研究旨在检测埃及银屑病患者中细胞因子基因TNF-α（-308）（G/A）、IL-10（-1082）（G/A）、IL-6（-174）（G/C）和IL-1Ra（VNTR）的多态性与银屑病的相关性。
本研究纳入了46例来自埃及尼罗河三角洲地区大学医院皮肤科的银屑病患者。其中包括14名男性和32名女性，年龄均值±标准差为46.68±12.16岁，年龄范围为15 - 70岁。将他们的基因型与来自同一地区年龄和性别匹配的98名健康对照进行比较。通过使用针对多态性等位基因的序列特异性引物进行聚合酶链反应（PCR）扩增脱氧核糖核酸来进行基因分型。
与对照组相比，病例组中某些基因型的频率显著更高，包括IL-6（-174）CC（P < 0.001，OR = 6.7）、IL-10（-1082）GG（P < 0.05，OR = 5.1）和TNF-α（-308）GG（P < 0.05，OR = 3.7）。在中度严重斑块亚型的病例中，TNF-α（-308）GG和IL-10（-1082）GG基因型的比例更高。IL-10 GA、IL-6 GC与TNF GA的联合杂合性在研究病例中的频率显著较低。
与IL6、IL10和TNF-α基因相关的基因多态性与银屑病呈现出特定的关联模式，这可能对疾病咨询和管理具有潜在影响。
