对流免疫电泳在巨细胞病毒抗体快速检测中的应用
Application of counterimmunoelectrophoresis in rapid detection of cytomegalovirus antibodies.
作者信息
Niebojewski R A, Aguilar-Torres F G, Rytel M W
出版信息
Am J Clin Pathol. 1977 Sep;68(3):343-6. doi: 10.1093/ajcp/68.3.343.
Determination of cytomegalovirus antibodies by counterimmune-electrophoresis correlated well with results of complement-fixation antibody studies. The sensitivity and specificity of the counterimmunoelectrophoresis method (vs. complement fixation) was 88.9%. Fourfold antibody increases detected by complement fixation were also detected by counterimmunoelectrophoresis. In addition, no cross-reaction was observed in the counterimmunoelectrophoresis assay between other DNA viral antisera and cytomegalovirus antigen (Ad-169).
通过对流免疫电泳法测定巨细胞病毒抗体，其结果与补体结合抗体研究结果高度相关。对流免疫电泳法（相对于补体结合法）的敏感性和特异性为88.9%。补体结合法检测到的四倍抗体增加也能被对流免疫电泳法检测到。此外，在对流免疫电泳试验中未观察到其他DNA病毒抗血清与巨细胞病毒抗原（Ad-169）之间的交叉反应。
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