一种用于乌昆耶米病毒和伦德病毒血清学的改良免疫电渗法的开发。
Development of a modified immunoelectroosmophoresis method for Uukuniemi and Runde virus serology.
作者信息
Traavik T
出版信息
Arch Virol. 1977;54(3):231-40. doi: 10.1007/BF01314789.
Abstract
In search for a suitable method for sero-ecological screenings for arboviruses in Norway, efforts were undertaken to make the immunoelectroosmophoresis technique more sensitive than here to fare in detection of antibodies. The aim was to make it comparable to haemagglutination inhibition test in sensitivity, retaining the advantages in specificity, simplicity and capacity. This has been achieved by: 1. Using concentrated virus as antigen. 2. Performing electrophoresis in a gel consisting of an agar-agarose mixture in optimal concentrations. 3. "Sandwiching" the first specific electrophoretic run with an anti-species antiserum to the tested sample.
摘要
为了寻找一种适合挪威虫媒病毒血清生态学筛查的方法，人们努力使免疫电渗技术比现有的方法更灵敏，以便在抗体检测中表现更好。目的是使其在灵敏度上与血凝抑制试验相当，同时保留特异性、简单性和检测能力方面的优势。这已通过以下方式实现：1. 使用浓缩病毒作为抗原。2. 在由最佳浓度的琼脂 - 琼脂糖混合物组成的凝胶中进行电泳。3. 用针对测试样品的抗种属抗血清“夹在”第一次特异性电泳过程中。
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2
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Am J Trop Med Hyg. 1958 Sep;7(5):561-73. doi: 10.4269/ajtmh.1958.7.561.
3
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