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采用补体结合试验(CF)、酶联免疫吸附测定(ELISA)和免疫印迹技术对巨细胞病毒抗体进行比较。

Comparison of cytomegalovirus antibodies by means of CF, ELISA and immunoblotting techniques.

作者信息

Jankowski M, Styczyński P

机构信息

Department of Virology, National Institute of Hygiene, Warsaw.

出版信息

Arch Immunol Ther Exp (Warsz). 1987;35(3):313-9.

PMID:2833198
Abstract

Antibody to human cytomegalovirus in sera of immune blood donors reacted with 12 viral polypeptides of molecular weight 200.000. 150.000, 65.000, 48.000, 34,000. 25.000, 23.000, 21.000 and 18.000. Development of CMV infection in renal transplant recipients resulted in increase of antibody reacting with various CMV polypeptides as well as in intensity of this reaction. The highly comparable results for CMV antibody detecting were obtained with immunoblotting and ELISA techniques.

摘要

免疫献血者血清中的人巨细胞病毒抗体与分子量为200,000、150,000、65,000、48,000、34,000、25,000、23,000、21,000和18,000的12种病毒多肽发生反应。肾移植受者中巨细胞病毒感染的发展导致与各种巨细胞病毒多肽发生反应的抗体增加以及这种反应的强度增加。用免疫印迹法和酶联免疫吸附测定技术获得了高度可比的巨细胞病毒抗体检测结果。

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