Vilariño M Luz, Le Guyader Françoise S, Polo David, Schaeffer Julien, Kröl Joanna, Romalde Jesús L
Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela, Spain.
Int Microbiol. 2009 Sep;12(3):145-51.
Standard and real-time reverse transcription-PCR (rRT-PCR) procedures were used to monitor cultured and wild bivalve molluscs from the Ría de Vigo (NW Spain) for the main human enteric RNA viruses, specifically, norovirus (NoV), hepatitis A virus (HAV), astrovirus (AsV), rotavirus (RT), enterovirus (EV), and Aichi virus (AiV). The results showed the presence of at least one enteric virus in 63.4% of the 41 samples analyzed. NoV GII was the most prevalent virus, detected in 53.7% of the samples, while NoV GI, AsV, EV, and RV were found at lower percentages (7.3, 12.2, 12.2, and 4.9%, respectively). In general, samples obtained in the wild were more frequently contaminated than those from cultured (70.6 vs. 58.3%) molluscs and were more readily contaminated with more than one virus. However, NoV GI was detected in similar amounts in cultured and wild samples (6.4 x 10(2) to 3.3 x 10(3) RNA copies per gram of digestive tissue) while the concentrations of NoV GII were higher in cultured (from 5.6 x 10(1) to 1.5 x 10(4) RNA copies per gram of digestive tissue) than in wild (from 1.3 x 10(2) to 3.4 x 10(4) RNA copies per gram of digestive tissue) samples.
采用标准实时逆转录聚合酶链反应（rRT-PCR）程序，对来自西班牙西北部维哥湾的养殖双壳贝类和野生双壳贝类进行监测，以检测主要的人类肠道RNA病毒，具体包括诺如病毒（NoV）、甲型肝炎病毒（HAV）、星状病毒（AsV）、轮状病毒（RT）、肠道病毒（EV）和爱知病毒（AiV）。结果显示，在分析的41个样本中，63.4%的样本至少含有一种肠道病毒。GII型诺如病毒是最常见的病毒，在53.7%的样本中被检测到，而GI型诺如病毒、AsV、EV和RV的检出率较低（分别为7.3%、12.2%、12.2%和4.9%）。总体而言，野生样本比养殖样本更容易受到污染（70.6%对58.3%），并且更容易被多种病毒污染。然而，GI型诺如病毒在养殖样本和野生样本中的检出量相似（每克消化组织中RNA拷贝数为6.4×10²至3.3×10³），而GII型诺如病毒在养殖样本中的浓度（每克消化组织中RNA拷贝数为5.6×10¹至1.5×10⁴）高于野生样本（每克消化组织中RNA拷贝数为1.3×10²至3.4×10⁴）。
