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检测和特征分析 2011 年泰国洪水中的肠病毒。

Detection and characterization of enteric viruses in flood water from the 2011 thai flood.

机构信息

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University.

出版信息

Jpn J Infect Dis. 2013;66(5):398-403. doi: 10.7883/yoken.66.398.

DOI:10.7883/yoken.66.398
PMID:24047738
Abstract

Severe flooding, which is associated with numerous outbreaks of a wide range of infectious diseases, particularly those caused by enteric viruses, occurred in all areas of Thailand in 2011. To determine the prevalence of five human enteric viruses, namely enterovirus, rotavirus (RV), norovirus (NV), hepatitis A virus (HAV), and hepatitis E virus, in the flood water, 100 water samples were collected from flood-damaged areas in central Thailand. Viral RNA was extracted from concentrated samples and analyzed by RT-PCR and sequencing. NV was the most commonly detected pathogen in the tested samples (14%). RV and HAV were detected in 9% and 7% of samples, respectively. This study is the first to detect enteric viral genes in flood water in Thailand. Furthermore, it is the first to detect an NV gene in any type of environmental water in Thailand. These results provide useful information for estimating the risk of flood waterborne viral infection.

摘要

2011 年,泰国所有地区都发生了严重的洪水灾害,由此引发了多种传染病的爆发,特别是肠道病毒引起的传染病。为了确定洪水水中五种人类肠道病毒(肠道病毒、轮状病毒、诺如病毒、甲型肝炎病毒和戊型肝炎病毒)的流行情况,从泰国中部受灾地区采集了 100 个水样。从浓缩样本中提取病毒 RNA,并通过 RT-PCR 和测序进行分析。在检测的样本中,NV 是最常见的病原体(14%)。RV 和 HAV 的检出率分别为 9%和 7%。本研究首次在泰国洪水水中检测到肠道病毒基因。此外,这也是首次在泰国任何类型的环境水中检测到 NV 基因。这些结果为评估洪水水源性病毒感染风险提供了有用信息。

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