维生素B12可防止氢氯噻嗪诱导的DNA损伤。
Vitamin B12 protects against DNA damage induced by hydrochlorothiazide.
作者信息
Alzoubi Karem H, Bayraktar Erva, Khabour Omar, Al-Azzam Sayer I
机构信息
Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.
出版信息
Saudi Pharm J. 2018 Sep;26(6):786-789. doi: 10.1016/j.jsps.2018.04.005. Epub 2018 Apr 3.
DNA damage induced by hydrochlorothiazide was previously reported in cultured human lymphocytes. In this study, we aimed to investigate the harmful effects of hydrochlorothiazide on DNA by measuring the spontaneous frequency of sister chromatid exchanges (SCEs) in cultured human lymphocytes. We also aimed to investigate the possible protection of that damage by vitamin B12. The results showed that hydrochlorothiazide (5 µg/mL) significantly increased the frequency of sister chromatid exchanges (P < 0.001) in human lymphocytes in comparison with control. Additionally, the frequency of hydrochlorothiazide-induced SCEs was significantly decreased by co-treatment with vitamin B12 at concentration of 13.5 µg/mL (P < 0.001). In conclusion, hydrochlorothiazide is genotoxic to human lymphocytes and its toxicity is reduced by vitamin B12.
先前有报道称，氢氯噻嗪可诱导培养的人淋巴细胞发生DNA损伤。在本研究中，我们旨在通过测量培养的人淋巴细胞中姐妹染色单体交换（SCE）的自发频率，来研究氢氯噻嗪对DNA的有害影响。我们还旨在研究维生素B12对这种损伤的可能保护作用。结果显示，与对照组相比，氢氯噻嗪（5μg/mL）显著增加了人淋巴细胞中姐妹染色单体交换的频率（P<0.001）。此外，在浓度为13.5μg/mL的维生素B12共同处理下，氢氯噻嗪诱导的SCE频率显著降低（P<0.001）。总之，氢氯噻嗪对人淋巴细胞具有遗传毒性，而维生素B12可降低其毒性。
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