Center of Technology, School of Chemistry (EQ), Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Foodborne Pathog Dis. 2010 Feb;7(2):217-9. doi: 10.1089/fpd.2009.0332.
This study aimed to verify the pasteurization efficiency and the microbiological quality of milk sold in the city of Rio de Janeiro, Brazil. The microbial quality of pasteurized milk samples was assessed by sample testing for the presence of Salmonella spp., coliforms at 35 degrees C, coliforms at 45 degrees C, and mesophilic bacterial counts. In addition, the pasteurization efficiency was verified through tests of neutral phosphatase and peroxidase enzymes. Salmonella spp. were not detected in any (100%) of the analyzed samples. However, 85 (70.8%) and 69 (57.5%) of the samples were noncompliant with current legal standards for coliforms at 35 degrees C and 45 degrees C, respectively. As for the aerobic mesophilic bacteria, 48 (40.0%) of the samples were noncompliant. From the 120 samples of pasteurized milk studied, 100% were negative for neutral phosphatase, whereas 12 (10.0%) were negative for peroxidase. Logistic regression indicated the absence of relationship between present lactoperoxidase and all the microbiological parameters studied, which suggested that the quality of pasteurized milk was associated with factors related to steps before or after heat treatment.
本研究旨在验证巴西里约热内卢市销售的巴氏奶的巴氏杀菌效率和微生物质量。通过检测巴氏奶样品中沙门氏菌、35°C 大肠菌群、45°C 大肠菌群和嗜温细菌计数来评估巴氏奶的微生物质量。此外,还通过中性磷酸酶和过氧化物酶的检测来验证巴氏杀菌效率。在分析的所有样本中均未检出沙门氏菌。然而,85 份(70.8%)和 69 份(57.5%)样本的 35°C 和 45°C 大肠菌群分别不符合现行法律标准。至于需氧嗜温菌,有 48 份(40.0%)样本不符合标准。在所研究的 120 份巴氏奶样本中,100%的样本对中性磷酸酶呈阴性反应,而 12 份(10.0%)样本对过氧化物酶呈阴性反应。逻辑回归表明,目前乳过氧化物酶与所有研究的微生物参数之间不存在关系,这表明巴氏奶的质量与热处理前后的相关因素有关。